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Titolo:
HETEROGENEITY IN MOLECULAR RECOGNITION BY RESTRICTION ENDONUCLEASES -OSMOTIC AND HYDROSTATIC-PRESSURE EFFECTS ON BAMHI, PVU-II, AND ECORV SPECIFICITY
Autore:
ROBINSON CR; SLIGAR SG;
Indirizzi:
UNIV ILLINOIS,SCH CHEM SCI URBANA IL 61801 UNIV ILLINOIS,SCH CHEM SCI URBANA IL 61801 UNIV ILLINOIS,BECKMAN INST ADV SCI & TECHNOL URBANA IL 61801
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 8, volume: 92, anno: 1995,
pagine: 3444 - 3448
SICI:
0027-8424(1995)92:8<3444:HIMRBR>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRYSTAL-STRUCTURE; NONSPECIFIC-BINDING; PROTEIN SOLVATION; BOUND WATER; DNA; REPRESSOR; DISSOCIATION; ASSOCIATION; COMPLEXES; STABILITY;
Keywords:
PROTEIN-DNA RECOGNITION; STAR ACTIVITY; BOUND WATER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
C.R. Robinson e S.G. Sligar, "HETEROGENEITY IN MOLECULAR RECOGNITION BY RESTRICTION ENDONUCLEASES -OSMOTIC AND HYDROSTATIC-PRESSURE EFFECTS ON BAMHI, PVU-II, AND ECORV SPECIFICITY", Proceedings of the National Academy of Sciences of the United Statesof America, 92(8), 1995, pp. 3444-3448

Abstract

The cleavage specificity of the Pvu II and BamHI restriction endonucleases is found to be dramatically reduced at elevated osmotic pressure. Relaxation in specificity of these otherwise highly accurate and specific enzymes, previously termed ''star activity,'' is uniquely correlated with osmotic pressure between 0 and 100 atmospheres. No other colligative solvent property exhibits a uniform correlation with star activity for all of the compounds tested. Application of hydrostatic pressure counteracts the effects of osmotic pressure and restores the natural selectivity of the enzymes for their canonical recognition sequences. These results indicate that water solvation plays an important role in the site-specific recognition of DNA by many restriction enzymes. Osmotic pressure did not induce an analogous effect on the specificity of the EcoRV endonuclease, implying that selective hydration effectsdo not participate in DNA recognition in this system. Hydrostatic pressure war; found to have little effect on the star activity induced bychanges in ionic strength, pH, or divalent cation, suggesting that distinct mechanisms may exist for these observed alterations in specificity. Recent evidence has indicated that BamHI and EcoRI share similar structural motifs, while Pvu II and EcoRV belong to a different structural family. Evidently, the use of hydration water to assist in site-specific recognition is a motif neither limited to nor defined by structural families.

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Documento generato il 27/09/20 alle ore 12:17:11