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Titolo:
THE FORCE-VELOCITY RELATIONSHIP OF THE ATP-DEPENDENT ACTIN-MYOSIN SLIDING CAUSING CYTOPLASMIC STREAMING IN ALGAL CELLS, STUDIED USING A CENTRIFUGE MICROSCOPE
Autore:
CHAEN S; INOUE J; SUGI H;
Indirizzi:
TEIKYO UNIV,SCH MED,DEPT PHYSIOL,ITABASHI KU TOKYO 173 JAPAN TEIKYO UNIV,SCH MED,DEPT PHYSIOL,ITABASHI KU TOKYO 173 JAPAN
Titolo Testata:
Journal of Experimental Biology
fascicolo: 4, volume: 198, anno: 1995,
pagine: 1021 - 1027
SICI:
0022-0949(1995)198:4<1021:TFROTA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
SINGLE KINESIN MOLECULES; CABLES INVITRO; INTERNODAL CELLS; CHARACEAN ALGAE; MUSCLE MYOSIN; MOVEMENT; NITELLA; BEADS; FILAMENTS; DONE;
Keywords:
FORCE-VELOCITY RELATIONSHIP; CYTOPLASMIC STREAMING; ALGA; ACTIN-MYOSIN SLIDING; CHARA CORALLINA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
S. Chaen et al., "THE FORCE-VELOCITY RELATIONSHIP OF THE ATP-DEPENDENT ACTIN-MYOSIN SLIDING CAUSING CYTOPLASMIC STREAMING IN ALGAL CELLS, STUDIED USING A CENTRIFUGE MICROSCOPE", Journal of Experimental Biology, 198(4), 1995, pp. 1021-1027

Abstract

When uncoated polystyrene beads suspended in Mg-ATP solution were introduced into the internodal cell of an alga Chara corallina, the beadsmoved along the actin cables with directions and velocities (30-62 mum s(-1)) similar to those of native cytoplasmic streaming. Bead movement was inhibited both in the absence of ATP and in the presence of Ca2+ as with native cytoplasmic streaming. These results indicate that bead movement is caused by cytoplasmic myosin molecules attached to thebead surface interacting with actin cables. The steady-state force-velocity relationship of the actin-myosin sliding that produces cytoplasmic streaming was determined by applying constant centrifugal forces to the beads moving on the actin cables. The force-velocity curve in the positive load region was nearly straight, and the implications of this shape are discussed in connection with the kinetic properties of the actin-myosin interaction in cytoplasmic streaming. It is suggested that the time for which a cytoplasmic myosin head is detached from actin in one cycle of actin-myosin interaction is very short. The Ca2+-induced actin-myosin linkages, responsible for the Ca2+-induced stoppage of cytoplasmic streaming, were shown to be much stronger than the rigor actin-myosin linkages.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 17:14:12