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Titolo:
SEQUENCE-ANALYSIS OF A GENE-CLUSTER INVOLVED IN METABOLISM OF 2,4,5-TRICHLOROPHENOXYACETIC ACID BY BURKHOLDERIA-CEPACIA AC1100
Autore:
DAUBARAS DL; HERSHBERGER CD; KITANO K; CHAKRABARTY AM;
Indirizzi:
UNIV ILLINOIS,COLL MED,DEPT MICROBIOL & IMMUNOL MC790,835 S WOLCOTT CHICAGO IL 60612 UNIV ILLINOIS,COLL MED,DEPT MICROBIOL & IMMUNOL MC790 CHICAGO IL 60612
Titolo Testata:
Applied and environmental microbiology
fascicolo: 4, volume: 61, anno: 1995,
pagine: 1279 - 1289
SICI:
0099-2240(1995)61:4<1279:SOAGII>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALCALIGENES-EUTROPHUS JMP134(PJP4); PSEUDOMONAS-CEPACIA; GLUTATHIONE-REDUCTASE; MOLECULAR CHARACTERIZATION; CATECHOL 1,2-DIOXYGENASES; EVOLUTIONARY DIVERGENCE; MALEYLACETATE REDUCTASE; TRICHOSPORON-CUTANEUM; STRUCTURAL GENE; PLASMID DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
66
Recensione:
Indirizzi per estratti:
Citazione:
D.L. Daubaras et al., "SEQUENCE-ANALYSIS OF A GENE-CLUSTER INVOLVED IN METABOLISM OF 2,4,5-TRICHLOROPHENOXYACETIC ACID BY BURKHOLDERIA-CEPACIA AC1100", Applied and environmental microbiology, 61(4), 1995, pp. 1279-1289

Abstract

Burkholderia cepacia AC1100 utilizes 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) as a sole source of carbon and energy. PT88 is a chromosomal deletion mutant of B. cepacia AC1100 and is unable to grow on 2,4,5-T. The nucleotide sequence of a 5.5-kb chromosomal fragment from B. cepacia AC1100 which complemented PT88 for growth on 2,4,5-T was determined. The sequence revealed the presence of six open reading frames, designated ORF1 to ORF6. Five polypeptides were produced when this DNA region was under control of the T7 promoter in Escherichia coli; however, no polypeptide was produced from the fourth open reading frame, ORF4. Homology searches of protein sequence databases were performed to determine if the proteins involved in 2,4,5-T metabolism were similar to other biodegradative enzymes, in addition, complementation studies were used to determine which genes were essential for the metabolism of 2,4,5-T. The first gene of the cluster, ORF1, encoded a 37-kDa polypeptide which was essential for complementation of PT88 and showed significant homology to putative trans-chlorodienelactone isomerases. The next gene, ORF2, was necessary for complementation and encoded a 47-kDa protein which showed homology to glutathione reductases. ORF3 was not essential for complementation; however, both the 23-kDa protein encoded by ORF3 and the predicted amino acid sequence of ORF4 shower homology to glutathione S-transferases. ORF5, which encoded an 11-kDa polypeptide, was essential for growth on 2,4,5-T, but the amino acid sequence did not show homology to those of any known proteins. The last geneof the cluster, ORF6, was necessary for complementation of PT88, and the 32-kDa protein encoded by this gene showed homology to catechol and chlorocatechol-1,2 dioxygenases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:04:22