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Titolo:
A NOVEL IN-VITRO SCREENING ASSAY FOR TRYPANOCIDAL ACTIVITY USING THE FLUORESCENT DYE BCECF-AM
Autore:
OBEXER W; SCHMID C; BRUN R;
Indirizzi:
SWISS TROP INST CH-4002 BASEL SWITZERLAND
Titolo Testata:
Tropical medicine and parasitology
fascicolo: 1, volume: 46, anno: 1995,
pagine: 45 - 48
SICI:
0177-2392(1995)46:1<45:ANISAF>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRYPANOSOMA-BRUCEI; INVITRO; EVANSI; EQUIPERDUM; RETENTION; RELEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
W. Obexer et al., "A NOVEL IN-VITRO SCREENING ASSAY FOR TRYPANOCIDAL ACTIVITY USING THE FLUORESCENT DYE BCECF-AM", Tropical medicine and parasitology, 46(1), 1995, pp. 45-48

Abstract

A cell viability assay, using fluorescence measurements has been developed for the screening of new compounds against African trypanosomes.l)-5(6)-carboxyfluorescein-pentaacetoxymethylester (BCECF-AM), an esterase substrate, was used in the assay as a marker for cell viability. Fluorescence was quantified using an automated fluorescence scanner for multi-well plates. Trypanosoma brucei rhodesiense, T. congolense, T. evansi and T. equiperdum from continuously growing cultures were exposed to various concentrations of trypanocidal drugs for an incubationperiod of 72 h at 37 degrees C. Then BCECF-AM was added to the cell suspensions and after 60 minutes the fluorescence of the trypanosome suspension was measured using the Millipore Cytofluor 2300 fluorescence scanner, at 485 nm excitation and 530 nm emission wavelengths. Resultsof kinetic studies of the hydrolysis of the non-fluorescent BCECF-AM in trypanosomes showed that BCECF-AM is readily cleaved by non-specific esterases to a highly fluorescent product. Drug concentrations causing 50 % inhibition of fluorescence (IC50-values) were measured fluorimetrically. Minimum inhibitory concentration (MIG) was determined microscopically.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 10:10:39