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Titolo:
OZONE-INDUCED AIRWAY HYPERRESPONSIVENESS - ROLE OF SUPEROXIDE ANIONS,NEP, AND BK RECEPTORS
Autore:
TSUKAGOSHI H; HADDAD EB; SUN JA; BARNES PJ; CHUNG KF;
Indirizzi:
ROYAL BROMPTON HOSP,NATL HEART & LUNG INST,DEPT THORAC MED,DOVEHOUSE ST LONDON SW3 6LY ENGLAND ROYAL BROMPTON HOSP,NATL HEART & LUNG INST,DEPT THORAC MED LONDON SW36LY ENGLAND
Titolo Testata:
Journal of applied physiology
fascicolo: 3, volume: 78, anno: 1995,
pagine: 1015 - 1022
SICI:
8750-7587(1995)78:3<1015:OAH-RO>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED BRONCHIAL HYPERREACTIVITY; KININ RECEPTORS; SUBSTANCE-P; GUINEA-PIGS; BRADYKININ; RESPONSES; EXPOSURE; PROTEIN; DOGS; INTERLEUKIN-1;
Keywords:
APOCYNIN; BROWN NORWAY RAT LUNGS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
H. Tsukagoshi et al., "OZONE-INDUCED AIRWAY HYPERRESPONSIVENESS - ROLE OF SUPEROXIDE ANIONS,NEP, AND BK RECEPTORS", Journal of applied physiology, 78(3), 1995, pp. 1015-1022

Abstract

We investigated the role of reactive oxygen species in ozone-induced airway hyperresponsiveness (AHR) in Brown Norway rats. Airway responsiveness to inhaled acetylcholine (ACh) and bradykinin (BK) and inflammatory cell recruitment in bronchoalveolar lavage fluid (BALF) were measured in vivo. Neutral endopeptidase (NEP) activity assay and measurement of BK-receptor binding sites in Brown Norway rat lungs were carriedout in vitro. Apocynin (5 mg/kg), an inhibitor of superoxide anion-generating NADPH oxidase, was administered perorally 30 min before a 3- or 6-h exposure to 3 ppm of ozone, and the animals were studied 18-24 h postexposure. Ozone induced increases in airway responsiveness to ACh and BK and in neutrophil counts in BALF. Apocynin inhibited the increase in airway responsiveness to BK but not to ACh without affecting the neutrophil counts in BALF. The antioxidants allopurinol and deferoxamine prevented ozone-induced AHR to both ACh and BK but did not reduce neutrophil counts. To further examine the mechanisms of ozone-induced AHR to BK, we measured NEP activity and the density of BK receptors in vitro after ozone exposure. Ozone exposure had no significant effect either on NEP activity or on the affinity and the number of BK receptors in lungs from rats treated with or without apocynin. We conclude that superoxide anions released from inflammatory cells in the airway may be involved in ozone-induced AHR. Inactivation of NEP or upregulation of BK receptors do not appear to be involved, but the possibility of localized changes cannot be excluded.

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Documento generato il 30/11/20 alle ore 16:14:08