Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
THE CRTA MOTIF IS PRESENT IN THE PROMOTERS OF MITOCHONDRIAL GENES OF RICE
Autore:
NAKAZONO M; TSUTSUMI N; HIRAI A;
Indirizzi:
UNIV TOKYO,FAC AGR,RADIAT GENET LAB,1-1-1 YAYOI TOKYO 113 JAPAN UNIV TOKYO,FAC AGR,RADIAT GENET LAB TOKYO 113 JAPAN
Titolo Testata:
PLANT SCIENCE
fascicolo: 2, volume: 105, anno: 1995,
pagine: 227 - 234
SICI:
0168-9452(1995)105:2<227:TCMIPI>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION INITIATION SITES; CAPPED INVITRO; ATP SYNTHASE; MAIZE; RNA; DNA; IDENTIFICATION; EXPRESSION; SUBUNIT-9;
Keywords:
IN VITRO CAPPING; MITOCHONDRION; RICE; CRTA MOTIF; TRANSCRIPTION INITIATION SITES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
M. Nakazono et al., "THE CRTA MOTIF IS PRESENT IN THE PROMOTERS OF MITOCHONDRIAL GENES OF RICE", PLANT SCI, 105(2), 1995, pp. 227-234

Abstract

In order to identify precisely the sites of initiation of transcription of mitochondrial genes of rice, we performed in vitro capping/ribonuclease protection experiments and primer extension analyses. Using this approach, we identified the transcription initiation sites of threegenes, namely, orf483, rrn18 and atp9. In previous studies, the transcription initiation sites of the rps3-rpl16-nad3-rps12 gene cluster and of two other genes, namely rrn26 and trnfM, were identified by the in vitro capping method. The alignment of sequences surrounding the initiation sites of these monocistronic and polycistronic transcripts revealed a conserved sequence motif. This conserved sequence exhibits a high degree of homology to the putative promoter sequence found in the mitochondrial DNAs (mtDNAs) of maize and wheat, an observation that suggests that this motif is recognized as a promoter in the rice mitochondria. In particular, the CRTA motif(where R is G or A) is highly conserved, as it is for example in maize, wheat, soybean and Oenothera. Toascertain the number of transcription initiation sites on the mitochondrial genome of rice, restriction fragments of rice mtDNA were allowed to hybridize with in vitro capped mitochondrial RNA and then were treated with ribonucleases T1 and A. The results of this hybridization experiment revealed that there are at least 16 sites of initiation of transcription on the mitochondrial genome of rice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 15:32:28