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Titolo:
UNIQUE SPECIFICITY OF IN-VITRO INHIBITION OF MOSQUITO MIDGUT TRYPSIN-LIKE ACTIVITY CORRELATES WITH IN-VIVO INHIBITION OF MALARIA PARASITE INFECTIVITY
Autore:
SHAHABUDDIN M; CRISCIO M; KASLOW DC;
Indirizzi:
NIAID,MALARIA RES LAB,MOLEC VACCINE SECT BETHESDA MD 20892
Titolo Testata:
Experimental parasitology
fascicolo: 2, volume: 80, anno: 1995,
pagine: 212 - 219
SICI:
0014-4894(1995)80:2<212:USOIIO>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
AEDES-AEGYPTI; PLASMODIUM-GALLINACEUM; TRANSMISSION; CULICIDAE; DIPTERA;
Keywords:
INHIBITION; TRYPSIN-LIKE ACTIVITY; MALARIA; PARASITE INFECTIVITY; MOSQUITO MIDGUT; AEDES AEGYPTI; PLASMODIUM GALLINACEUM; PERITROPHIC MATRIX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
M. Shahabuddin et al., "UNIQUE SPECIFICITY OF IN-VITRO INHIBITION OF MOSQUITO MIDGUT TRYPSIN-LIKE ACTIVITY CORRELATES WITH IN-VIVO INHIBITION OF MALARIA PARASITE INFECTIVITY", Experimental parasitology, 80(2), 1995, pp. 212-219

Abstract

Synchrony in the egress of Plasmodium ookinetes from the food bolus and enzymatic digestion of the blood meal in the mosquito midgut suggests that digestive enzymes play a role in the successful transmission of malaria parasites. Previously, we found that parasite-produced chitinase is essential for parasite transmission and can be activated by mosquito midgut protease. To determine the suitability of developing a transmission-blocking vaccine directed against mosquito trypsinlike enzyme(s), Aedes aegypti midgut trypsin-like proteases were characterizedbiochemically and compared to a mammalian trypsin. Mosquito trypsin is more sensitive to inhibition by aprotinin and less sensitive to egg white trypsin inhibitor than is bovine pancreatic trypsin. Soybean trypsin inhibitor and leupeptin inhibit both enzymes to similar extent. Membrane-feeding assays with aprotinin, leupeptin, and egg white trypsin inhibitor revealed a correlation between in vitro inhibition of mosquito trypsin-like activity and transmission-blocking activity; The results suggest a role for mosquito midgut trypsin(s) in malaria parasitedevelopment and indicate that the protease(s) is a potential target for blocking malaria transmission. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:59:51