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Titolo:
A BASIC TAIL INCREASES REPRESSION BY DIMERIC LAC REPRESSOR
Autore:
KOLKHOF P; OEHLER S; ALEX R; MULLERHILL B;
Indirizzi:
UNIV COLOGNE,INST GENET,WEYERTAL 121 D-50931 COLOGNE GERMANY UNIV COLOGNE,INST GENET D-50931 COLOGNE GERMANY
Titolo Testata:
Journal of Molecular Biology
fascicolo: 3, volume: 247, anno: 1995,
pagine: 396 - 403
SICI:
0022-2836(1995)247:3<396:ABTIRB>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; DNA LOOP; PROTEIN; BINDING; OPERATORS; TRANSCRIPTION; SITES; SEQUENCES; COMPLEXES; MULTIPLE;
Keywords:
PROTEIN-DNA RECOGNITION; DNA LOOP FORMATION; BASIC TAIL; REPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
P. Kolkhof et al., "A BASIC TAIL INCREASES REPRESSION BY DIMERIC LAC REPRESSOR", Journal of Molecular Biology, 247(3), 1995, pp. 396-403

Abstract

Tetrameric Lac repressor achieves cooperative repression by binding simultaneously to O1 and to one of the auxiliary operators O2 or O3, thereby forcing the intervening DNA into a loop. Dimeric Lac repressor is not able to form DNA loops and consequently shows no cooperative repression. We constructed a dimeric Lac repressor mutant which exhibits increased repression to the Inc operon that does not depend on specific operator-repressor-operator loops. This Lac repressor carries a synthetic tail of basic residues attached to its C terminus. With this construct, we observe an increase of the in vivo repression upon additionof auxiliary Inc operators to a chromosomal lac operon controlled by O1. This suggests that the basic tail enables dimeric Lac repressor toenhance its repression by additional non-specific DNA contacts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 00:44:12