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Titolo:
APOPTOSIS (PROGRAMMED CELL-DEATH) IN ARTERIES OF THE NEONATAL LAMB
Autore:
CHO A; COURTMAN DW; LANGILLE BL;
Indirizzi:
TORONTO HOSP,RES INST,BANTING & BEST DIABET CTR,200 ELIZABETH ST TORONTO ON M5G 2C4 CANADA TORONTO HOSP,RES INST,BANTING & BEST DIABET CTR TORONTO ON M5G 2C4 CANADA UNIV TORONTO,DEPT PATHOL TORONTO ON CANADA
Titolo Testata:
Circulation research
fascicolo: 2, volume: 76, anno: 1995,
pagine: 168 - 175
SICI:
0009-7330(1995)76:2<168:A(CIAO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEXAMETHASONE-INDUCED APOPTOSIS; BLOOD-FLOW; INTERNUCLEOSOMAL FRAGMENTATION; EPITHELIAL-CELLS; PERINATAL-PERIOD; GENE CED-3; DNA; INJURY; PROLIFERATION; THYMOCYTES;
Keywords:
CELL SUICIDE; PHYSIOLOGICAL CELL DEATH; ARTERIAL REMODELING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
A. Cho et al., "APOPTOSIS (PROGRAMMED CELL-DEATH) IN ARTERIES OF THE NEONATAL LAMB", Circulation research, 76(2), 1995, pp. 168-175

Abstract

We have examined whether cell death contributes to postnatal remodeling of arteries in lambs. First, abdominal aortic smooth muscle cell proliferation rates fell from 2.87+/-0.08% per day at 3 days of age to 1.75+/-0.15% per day at 21 days. These proliferation rates would yield a 50% increase in DNA content in the absence of cell death. No increase in DNA content was observed (P<.05 for predicted versus measured accumulation); therefore, significant cell death was inferred. The same analysis did not indicate high cell-death rates in the carotid, renal, or iliac arteries; however, cell death was detected in situ by end-labeling partially degraded DNA with terminal deoxynucleotidyl transferase or by nuclear labeling with propidium iodide, a fluorescent dye thatpermeates only nonviable cells. Nuclei were labeled in all arteries, although labeling was most frequent in the abdominal aorta, a vessel that regresses substantially after birth. Cell death was apoptotic because DNA extracted from arteries and end-labeled with [P-31]dCTP produced a series of low molecular weight bands (DNA ladder) on an agarose gel, a hallmark of apoptosis. The ladder was strong for neonatal abdominal aorta but weak for other arteries. Only weak laddering was observed for fetal abdominal aortas in late gestation, confirming that high apoptosis rates in this vessel were initiated after birth. Intense DNA ladders and frequent in situ labeling indicated high rates of apoptosis in the postnatal intra-abdominal umbilical artery, another vessel that regresses after birth. We conclude that apoptosis contributes to postpartum arterial remodeling. This contribution is greatest in arteries that regress after birth.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 08:20:39