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Titolo:
MOUSE MAMMARY-TUMORS EXPRESS ELEVATED LEVELS OF RNA-ENCODING THE MURINE HOMOLOG OF SKY, A PUTATIVE RECEPTOR TYROSINE KINASE
Autore:
TAYLOR ICA; ROY S; YASWEN P; STAMPFER MR; VARMUS HE;
Indirizzi:
NCI,BLDG 49,RM 4A56,9000 ROCKVILLE PIKE BETHESDA MD 20892 NCI BETHESDA MD 20892 UNIV CALIF SAN FRANCISCO,DEPT MICROBIOL & IMMUNOL SAN FRANCISCO CA 94143 UNIV CALIF BERKELEY,LAWRENCE BERKELEY LAB BERKELEY CA 94720
Titolo Testata:
The Journal of biological chemistry
fascicolo: 12, volume: 270, anno: 1995,
pagine: 6872 - 6880
SICI:
0021-9258(1995)270:12<6872:MMEELO>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-BREAST-CANCER; PP60C-SRC PROTEIN-KINASE; EPITHELIAL-CELL LINE; SIGNAL TRANSDUCTION; TRANSGENIC MICE; GROWTH-FACTOR; GENE; ONCOGENE; TRANSFORMATION; VIRUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
I.C.A. Taylor et al., "MOUSE MAMMARY-TUMORS EXPRESS ELEVATED LEVELS OF RNA-ENCODING THE MURINE HOMOLOG OF SKY, A PUTATIVE RECEPTOR TYROSINE KINASE", The Journal of biological chemistry, 270(12), 1995, pp. 6872-6880

Abstract

To gain insight into the signal transduction pathways utilized by theWnt-1-responsive mammary epithelial cell line C57MG, we screened for non-src family member tyrosine kinases expressed in these cells using a polymerase chain reaction-based technique. We identified five cDNA clones encoding receptor tyrosine kinases for which the ligand is known(fibroblast growth factor receptor, platelet-derived growth factor receptor, epithelial growth factor receptor, insulin receptor, and insulin-like growth factor receptor), two putative receptor tyrosine kinases for which the ligand remains to be identified (the products of ryk and the mouse klg homolog), and a novel tyrosine kinase. We cloned cDNAs encoding both the murine and human homologs of this kinase, the sequences of which were subsequently published under the names sky (Ohashi, K., Mizuno, K., Kuma, K., Miyata, T., and Nakamura, T. (1994) Oncogene 9, 699-705) and rse (Mark, M. R., Scadden, D. T., Wang, Z., Gu, Q.,Goddard, A., and Godowski, P. J. (1994) J. Biol. Chem. 269, 10720-10728). Mouse sky RNA levels are abundant in mammary tumors derived from transgenic mice that express wnt-1, fgf-3, or both oncogenes in their mammary glands. However, little or no expression of sky is detected inmammary glands from virgin animals or in preneoplastic mammary glandsfrom wnt-1 transgenic mice. Moreover, we find that the human homolog of sky is expressed at elevated levels when normal human mammary epithelial cells are rendered tumorigenic by the introduction of two viral oncogenes. Transient transfection of the human SKY cDNA into the quailfibrosarcoma cell line QT6 reveals that SKY is an active tyrosine kinase that augments the level of cellular phosphotyrosine. Introduction of murine Sky into RatB1a fibroblasts by retrovirus-mediated gene transfer results in morphological transformation, growth in soft agar, andthe formation of tumors in nude mice. These data raise the possibility that the Sky tyrosine kinase is involved in the development and/or progression of mammary tumors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 21:37:52