Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EFFECT OF OROTIC-ACID ON TGF-BETA-1-INDUCED GROWTH-INHIBITION OF L1210 LEUKEMIC-CELLS
Autore:
GRZELKOWSKA K; MOTYL T; MALICKA E; OSTROWSKI J; TRZECIAK L; FILIPECKI M;
Indirizzi:
AGR UNIV WARSAW,FAC VET MED,DEPT ANIM PHYSIOL,NOWOURSYNOWSKA 166 PL-02766 WARSAW POLAND AGR UNIV WARSAW,FAC VET MED,DEPT ANIM PHYSIOL PL-02766 WARSAW POLAND
Titolo Testata:
International journal of hematology
fascicolo: 1, volume: 61, anno: 1995,
pagine: 23 - 33
SICI:
0925-5710(1995)61:1<23:EOOOTG>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Keywords:
OROTIC ACID; TGF-BETA-1; ORNITHINE DECARBOXYLASE; L1210; LEUKEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
NO
Recensione:
Indirizzi per estratti:
Citazione:
K. Grzelkowska et al., "EFFECT OF OROTIC-ACID ON TGF-BETA-1-INDUCED GROWTH-INHIBITION OF L1210 LEUKEMIC-CELLS", International journal of hematology, 61(1), 1995, pp. 23-33

Abstract

Transforming growth factor-beta 1 (TGF-beta 1) exerted growth-inhibitory effect on L1210 leukemic cell line, manifested by the decrease in viable and increase in dead cells, The cell death evoked by TGF-beta 1was both necrotic and apoptotic, quantified by the trypan blue exclusion method and apoptotic index, respectively. The induction of programmed cell death by TGF-beta 1 was confirmed by gel electrophoresis of DNA, where the characteristic 'DNA ladder' resulting from the internucleosomal DNA cleavage was visualized. The enhancement of cell mortalityby TGF-beta 1 was associated with the inhibition of ornithine decarboxylase (ODC) expression (measured by the reverse transcriptase-polymerase chain reaction method) and impaired activity of this key enzyme inpolyamine synthesis, Orotic acid (OA) - a known tumor promoter - stimulated proliferation of L1210 leukemic cells and diminished the necrotic effect of TGF-beta 1, but it did not change the extent of apoptosisevoked by TGF-beta 1. OA increased the expression of ODC and diminished depressional influence of TGF-beta 1 on transcription and activity of ODC in leukemic cells. In conclusion: OA is a bioactive compound stimulating the growth of leukemic cells and diminishing the growth-inhibitory effect of TGF-beta 1. ODC gene is probably one of the targets for both OA and TGF-beta 1 influences in L1210 leukemic cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 03:21:21