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Titolo:
TRAPPING, DETECTION, AND MASS DETERMINATION OF COLIPHAGE T4 DNA IONS OF 10(8) DA BY ELECTROSPRAY-IONIZATION FOURIER-TRANSFORM ION-CYCLOTRONRESONANCE MASS-SPECTROMETRY
Autore:
CHEN RD; CHENG XH; MITCHELL DW; HOFSTADLER SA; WU QY; ROCKWOOD AL; SHERMAN MG; SMITH RD;
Indirizzi:
BATTELLE MEM INST,PACIFIC NW LABS,DEPT CHEM SCI,POB 999,MS P8-19 RICHLAND WA 99352 BATTELLE MEM INST,PACIFIC NW LABS,DEPT CHEM SCI RICHLAND WA 99352 BATTELLE MEM INST,PACIFIC NW LABS,ENVIRONM & MOLEC SCI LAB RICHLAND WA 99352
Titolo Testata:
Analytical chemistry
fascicolo: 7, volume: 67, anno: 1995,
pagine: 1159 - 1163
SICI:
0003-2700(1995)67:7<1159:TDAMDO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
LARGE MOLECULES; BIOMOLECULES; SIGNAL; MOTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
R.D. Chen et al., "TRAPPING, DETECTION, AND MASS DETERMINATION OF COLIPHAGE T4 DNA IONS OF 10(8) DA BY ELECTROSPRAY-IONIZATION FOURIER-TRANSFORM ION-CYCLOTRONRESONANCE MASS-SPECTROMETRY", Analytical chemistry, 67(7), 1995, pp. 1159-1163

Abstract

The trapping, detection, and mass measurement of individual T4 DNA ions (nominal molecular weight, M(r) = 1.1 x 10(8) Da) have been performed with a 7 T electrospray ionization Fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometer. The ionic mass was obtained by direct measurement of the number of charges carried by individual T4DNA ions. The ions detected are a factor of similar to 20 larger thanany molecule previously studied by mass spectrometry. These gas-phasemacroions demonstrate a high degree of stability, which allows the acquisition of long transients (greater than or equal to 476 s) from individual ions and the study of relatively slow reactions or dissociation processes. These results show the potential utility of ESI-FTICR mass spectrometry for the analysis of very large biomolecules and microparticles and indicate that it is possible to transfer ions of 110 MDa size to the gas phase substantially intact. The potential applications of the FTICR analysis of such highly charged ions are briefly discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 13:12:10