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Titolo:
DITHIOTHREITOL AND COBALT EFFECTS ON MEMBRANE-ASSOCIATED PEROXIDASES OXIDIZING FERULOYL-COA
Autore:
MYTON KE; FRY SC;
Indirizzi:
UNIV EDINBURGH,DIV BIOL SCI,DANIEL RUTHERFORD BLDG,KINGS BLDG,MAYFIELD RD EDINBURGH EH9 3JH MIDLOTHIAN SCOTLAND UNIV EDINBURGH,DIV BIOL SCI EDINBURGH EH9 3JH MIDLOTHIAN SCOTLAND
Titolo Testata:
Phytochemistry
fascicolo: 3, volume: 38, anno: 1995,
pagine: 573 - 577
SICI:
0031-9422(1995)38:3<573:DACEOM>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-WALL; DIFERULIC ACID; LIGNIN; POLYSACCHARIDES; ISOPEROXIDASES; COLEOPTILES; HYPOCOTYLS; COMPONENT; LINKAGES; OXIDASE;
Keywords:
ZEA MAYS; POACEAE; MAIZE; ENDO-MEMBRANE ISOLATION; FERULOYL-COA METABOLISM; PEROXIDASE; THIOLS; COBALT; FERULOYLTRANSFERASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
K.E. Myton e S.C. Fry, "DITHIOTHREITOL AND COBALT EFFECTS ON MEMBRANE-ASSOCIATED PEROXIDASES OXIDIZING FERULOYL-COA", Phytochemistry, 38(3), 1995, pp. 573-577

Abstract

Membrane preparations from cell-suspension cultures of maize producedC-14-polymeric material from [methoxy-C-14]feruloyl-CoA under conditions reported to be suitable for the assay of feruloyl-CoA: polysaccharide feruloyltransferase activity. The major C-14-polymer formed did not contain O-[C-14]feruloyl-arabinofuranosyl groups. Production of C-polymer was prevented by catalase, indicating a requirement for endogenous H2O2, and was Co2+-dependent but only in the presence of dithiothreitol (DTT). The membranes exhibited peroxidase activity with guaiacol and H2O2; this reaction was blocked by DTT. It is concluded that peroxidases and H2O2, endogenous to the isolated membranes, caused oxidative polymerization of [C-14]feruloyl groups of feruloyl-CoA; DTT prevented polymerization by acting as a competitive substrate for peroxidases, and Co2+ restored polymerization by forming an insoluble complex with the thiol.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 18:28:52