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Titolo:
UPTAKE AND METABOLISM OF BORONOPHENYLALANINE IN HUMAN UVEAL MELANOMA-CELLS IN CULTURE - RELEVANCE TO BORON NEUTRON-CAPTURE THERAPY OF CANCER-CELLS
Autore:
BELKHOU R; ABBE JC; PHAM P; JASNER N; SAHEL J; DREYFUS H; MOUTAOUAKKIL M; MASSARELLI R;
Indirizzi:
HOP MICHALLON,INSERM,U318,BP 217X F-38043 GRENOBLE FRANCE CTR RECH NUCL,CHIM NUCL LAB,CNRS F-67037 STRASBOURG FRANCE CEA,DEPT MOLEC MARQUEES SACLAY FRANCE HUS,OPHTALMOL CLIN,INSERM,CJF 9202 STRASBOURG FRANCE UNIV M BEN ABDALLAH,FAC SCI,BIOCHIM LAB FES MOROCCO
Titolo Testata:
Amino acids
fascicolo: 2, volume: 8, anno: 1995,
pagine: 217 - 229
SICI:
0939-4451(1995)8:2<217:UAMOBI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACIDS; MICE;
Keywords:
AMINO ACIDS; HUMAN; UVEAL; MELANOMA CULTURES; BOROPHENYLALANINE; TRANSPORT KINETICS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
R. Belkhou et al., "UPTAKE AND METABOLISM OF BORONOPHENYLALANINE IN HUMAN UVEAL MELANOMA-CELLS IN CULTURE - RELEVANCE TO BORON NEUTRON-CAPTURE THERAPY OF CANCER-CELLS", Amino acids, 8(2), 1995, pp. 217-229

Abstract

The transport of boronophenylalanine (BPA) and its metabolic fate have been studied in a human uveal melanoma cell line isolated from a primary enucleated tumor. The boronated compound was rapidly incorporatedinto the cells reaching a peak of incorporation in two hours. This was followed by a trough between 10 and 24 hours and by an increase thereafter. The analogy with the amino acids phenylalanine (Phe) and tyrosine (Tyr) was studied in competition experiments incubating cultures of cell line MK-T isolated in this laboratory, with [H-3]-Phe and [I-125]-Tyr, in the presence or absence of various concentrations of BPA, between 0 and 5 min. The presence of BPA severely reduced the uptake ofboth amino acids. The kinetics of the transport of [H-3]-Phe and [H-3]-Tyr in the presence of BPA, measured after 10 sec of incubation, showed that the boronated compound exerted a competitive inhibition on both transport systems. The intracellular metabolism of BPA was followedby measuring boron concentration (measured with Ionization Coupled Mass Spectrometry) in subcellular fractions and after membrane extraction by the detergent Triton X-100. The results showed that BPA remained in the supernatant and was not metabolized into macromolecules. These results and the relative absence of melanine in these cells, as observed by electron microscopy, suggest that BPA may be actively transported into melanoma cells but not metabolized. The results may have a relevance in studies on Boron Neutron Capture Therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 15:41:57