Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IN-VITRO INFLUENCE OF PLASMA STEROID-BINDING PROTEINS ON ANDROGEN METABOLISM IN HUMAN-LEUKOCYTES
Autore:
DECHAUD H; GOUJON R; CLAUSTRAT F; BOUCHERAT M; PUGEAT M;
Indirizzi:
HOP ANTIQUAILLE,HOSPICES CIVILS LYON,CENT BIOCHIM LAB,1 RUE ANTIQUAILLE F-69321 LYON 05 FRANCE HOP ANTIQUAILLE,HOSPICES CIVILS LYON,CLIN ENDOCRINOL LAB LYON FRANCE HOP DEBROUSSE,INSERM,U329 F-69322 LYON FRANCE
Titolo Testata:
Steroids
fascicolo: 2, volume: 60, anno: 1995,
pagine: 226 - 233
SICI:
0039-128X(1995)60:2<226:IIOPSP>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
FREE HORMONE HYPOTHESIS; TESTOSTERONE; GLOBULIN; TRANSPORT; CELLS; ESTRADIOL; SERUM; SKIN;
Keywords:
ANDROGEN METABOLISM; STEROID-BINDING PROTEINS; SEX HORMONE BINDING-GLOBULIN (SHBG); 5-ALPHA-REDUCTASE; LEUKOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
H. Dechaud et al., "IN-VITRO INFLUENCE OF PLASMA STEROID-BINDING PROTEINS ON ANDROGEN METABOLISM IN HUMAN-LEUKOCYTES", Steroids, 60(2), 1995, pp. 226-233

Abstract

The purpose of this study was to investigate the influence of plasma steroid-binding proteins on androgen metabolism in intact leukocytes prepared from normal male and female blood samples. Leukocyte preparations were incubated for 24 h at 37 degrees C with either labeled or unlabeled testosterone (T), 5 alpha-dihydrotestosterone (5 alpha-DHT), and androstenedione (A). After extraction, the formed labeled metabolites were first identified by high performance liquid chromatography, then, using unlabeled substrates, metabolite concentrations were measuredby specific radioimmunoassays. The conversion ratios of substrate to metabolite were calculated for each preparation using either labeled or unlabeled substrates. In the absence of steroid-binding proteins, the mean conversion ratios of T to A, A to T, T to 5 alpha-DHT, and 5 alpha-DHT to 3 alpha-androstanediol (3 alpha-D) were, in males and females, respectively, 5.6% and 6.1% (n = 11), 5.6% and 5.6% (n = 5), 2.8% and 2.2% (n = 11), 43.1% and 40.0% (n = 5), these sex differences being non-significant. The presence of increasing amounts of plasma, purified albumin or sex hormone binding-globulin (SHBG) in the incubation media reduced metabolite formation dose-dependently. However, a 1000-fold greater concentration of albumin than of SHBG was necessary for 50%inhibition of androgen metabolism by leukocytes, showing SHBG to havethe main protective effect. Moreover, in the presence of various concentrations of T or 5 alpha-DHT, and of albumin or SHBG, metabolite formation was positively and highly significantly correlated with the concentration of protein-unbound (free) substrate measured by equilibriumdialysis (r = 0.964 for conversion of T to A and r = 0.998 for conversion of 5 alpha-DHT to 3 alpha-D) but weakly correlated with total substrate concentration (r = 0.375 for total T and r = 0.669 for total 5 alpha-DHT). Additionally, leukocyte metabolism of 5 alpha-DHT was significantly enhanced when free 5 alpha-DHT levels increased in the presence of 17 beta-estradiol (which displaced 5 alpha-DHT from the SHBG-binding sites). We conclude that the metabolism of androgens by human leukocytes is mainly regulated by SHBG level, in both men and women. Although the rate of androgen metabolism by leukocytes was not determinedin this study, reduced serum SHBG levels with unchanged albumin levels or drugs capable of displacing androgens from serum SHBG can be expected to increase androgen metabolism in human leukocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 19:46:58