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Titolo:
ISOLATION OF PURE POPULATIONS OF EPITHELIAL AND MYOEPITHELIAL CELLS FROM THE NORMAL HUMAN MAMMARY-GLAND USING IMMUNOMAGNETIC SEPARATION WITH DYNABEADS
Autore:
GOMM JJ; BROWNE PJ; COOPE RC; LIU QY; BULUWELA L; COOMBES RC;
Indirizzi:
UNIV LONDON,CHARING CROSS & WESTMINSTER MED SCH,DEPT MED ONCOL,ST DUNSTANS RD LONDON W6 8RP ENGLAND UNIV LONDON,CHARING CROSS & WESTMINSTER MED SCH,DEPT BIOCHEM LONDON W6 8RP ENGLAND
Titolo Testata:
Analytical biochemistry
fascicolo: 1, volume: 226, anno: 1995,
pagine: 91 - 99
SICI:
0003-2697(1995)226:1<91:IOPPOE>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-BONE MARROW; MONOCLONAL-ANTIBODIES; MAGNETIC MICROSPHERES; NEGATIVE SELECTION; POSITIVE SELECTION; BREAST-CANCER; PURIFICATION; PARTICLES; CULTURE; GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
J.J. Gomm et al., "ISOLATION OF PURE POPULATIONS OF EPITHELIAL AND MYOEPITHELIAL CELLS FROM THE NORMAL HUMAN MAMMARY-GLAND USING IMMUNOMAGNETIC SEPARATION WITH DYNABEADS", Analytical biochemistry, 226(1), 1995, pp. 91-99

Abstract

Monoclonal antibodies to epithelial membrane antigen and common acutelymphoblastic leukemia antigen were bound to second antibody-coated magnetic microspheres. These specific antibody/bead complexes were thenused to directly isolate purified epithelial and myoepithelial cells from normal breast organoid cell preparations by magnetic separation. Near homogeneous cell populations were selected with yields of 10-20 X10(6) epithelial and myoepithelial cells per organoid preparation. Repeated purification steps allowed almost complete depletion of myoepithelial cells for RNA studies. Tissue culture of separated cell populations in appropriate defined media further ensured purity of cell type and was unimpeded by Dynabead attachment. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 04:39:56