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Titolo:
DIAGNOSIS OF ASTROVIRUS BY REVERSE-TRANSCRIPTASE PCR IN STOOLS FROM PATIENTS IN THE UK AND SAUDI-ARABIA
Autore:
AKHTER J; QADRI SMH; MYINT SH;
Indirizzi:
UNIV LEICESTER,DEPT MICROBIOL & IMMUNOL,MED SCI BLDG,UNIV RD LEICESTER LE1 7RH ENGLAND UNIV LEICESTER,DEPT MICROBIOL & IMMUNOL,MED SCI BLDG,UNIV RD LEICESTER LE1 7RH ENGLAND KING FAISAL HOSP & RES CTR RIYADH SAUDI ARABIA
Titolo Testata:
Clinical and diagnostic virology
fascicolo: 3, volume: 3, anno: 1995,
pagine: 215 - 220
SICI:
0928-0197(1995)3:3<215:DOABRP>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFANTILE GASTROENTERITIS; CHILDREN; SEROTYPE-1; DIARRHEA;
Keywords:
ASTROVIRUS; PCR; DIARRHEA; SAUDI-ARABIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
J. Akhter et al., "DIAGNOSIS OF ASTROVIRUS BY REVERSE-TRANSCRIPTASE PCR IN STOOLS FROM PATIENTS IN THE UK AND SAUDI-ARABIA", Clinical and diagnostic virology, 3(3), 1995, pp. 215-220

Abstract

Background: Astroviruses are becoming increasingly recognised as a cause of diarrhoea in children, and to a lesser extent, adults. Electronmicroscopy is the recognised standard for diagnosis but is not available in many centres. Objectives: This study set out to evaluate a RT-PCR method for the diagnosis of astrovirus in faecal specimens. Study design: 130 diarrhoeal stools were collected prospectively both from Saudi Arabia and Leicester, UK. The patients from each centre were age-matched. Twenty control isolates from food service staff without clinical illness were also collected. Both electron microscopy (EM) and RT-PCR (for astrovirus serotype 1) were used to diagnose astrovirus infection. Results: Of 23 PCR-positive specimens, only 17 were detected by EM. The prevalence of astrovirus serotype 1 was determined by PCR as 1.5% in Saudi Arabia and 4.6% in the UK. Conclusion: RT-PCR is a more sensitive alternative to EM for astrovirus serotype 1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 07:06:07