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Titolo:
EVALUATION OF OPTIMAL VOLTAGE-SENSITIVE DYES FOR OPTICAL MONITORING OF EMBRYONIC NEURAL ACTIVITY
Autore:
MOMOSESATO Y; SATO K; SAKAI T; HIROTA A; MATSUTANI K; KAMINO K;
Indirizzi:
TOKYO MED & DENT UNIV,SCH MED,DEPT PHYSIOL,BUNKYO KU TOKYO 113 JAPAN TOKYO MED & DENT UNIV,SCH MED,DEPT PHYSIOL,BUNKYO KU TOKYO 113 JAPAN NIPPON KANKOH SHIKISO KENKYUSHO OKAYAMA 700 JAPAN
Titolo Testata:
The Journal of membrane biology
fascicolo: 2, volume: 144, anno: 1995,
pagine: 167 - 176
SICI:
0022-2631(1995)144:2<167:EOOVDF>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHICK BRAIN-STEM; CENTRAL NERVOUS-SYSTEM; ELECTRICAL-ACTIVITY; VAGAL-STIMULATION; POTENTIOMETRIC PROBES; FLUORESCENT-PROBES; AXON FLUORESCENCE; NEURONAL-ACTIVITY; ACTION-POTENTIALS; APLYSIA NEURONS;
Keywords:
OPTICAL MONITORING; VOLTAGE-SENSITIVE DYES; MEROCYANINE-RHODANINES; EMBRYONIC NEURAL ACTIVITY; EMBRYONIC NERVOUS TISSUES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
Y. Momosesato et al., "EVALUATION OF OPTIMAL VOLTAGE-SENSITIVE DYES FOR OPTICAL MONITORING OF EMBRYONIC NEURAL ACTIVITY", The Journal of membrane biology, 144(2), 1995, pp. 167-176

Abstract

To evaluate the suitability of a variety of fast voltage-sensitive dyes for optical recording of rapid transmembrane potential activity in the embryonic nervous system, we screened over twenty dyes, including several newly synthesized probes, in three different embryonic neural preparations: cervical vagus nerve bundle, nodose ganglion, and brainstem from 7-day old chick embryos. Measurements of voltage-related optical signals were made using a multiple-site optical recording system. Signal size, signal-to-noise ratio, photobleaching, and phototoxicity were examined. Several promising new merocyanine-rhodanine dyes for embryonic nervous systems were found.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 19:38:52