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Titolo:
PREMATURE CHROMOSOME CONDENSATION OF THE KARYOGAMY-BLOCKED SPERM PRONUCLEUS IN THE FERTILIZATION OF FUCUS-DISTICHUS (FUCALES, PHAEOPHYCEAE)
Autore:
MOTOMURA T;
Indirizzi:
HOKKAIDO UNIV,INST ALGOL RES MURORAN HOKKAIDO 051 JAPAN
Titolo Testata:
Journal of phycology
fascicolo: 1, volume: 31, anno: 1995,
pagine: 108 - 113
SICI:
0022-3646(1995)31:1<108:PCCOTK>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMALIAN CELL FUSION; DNA-SYNTHESIS; FUCOID ALGAE; PELVETIA; POLYSPERMY; MATURATION; INDUCTION; OOCYTES; CYCLE;
Keywords:
COLCHICINE; FERTILIZATION; FUCUS DISTICHUS; KARYOGAMY MICROSPECTROPHOTOMETRY; PHAEOPHYCEAE; PREMATURE CHROMOSOME CONDENSATION; SPINDLE FORMATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
T. Motomura, "PREMATURE CHROMOSOME CONDENSATION OF THE KARYOGAMY-BLOCKED SPERM PRONUCLEUS IN THE FERTILIZATION OF FUCUS-DISTICHUS (FUCALES, PHAEOPHYCEAE)", Journal of phycology, 31(1), 1995, pp. 108-113

Abstract

Mitosis of egg and sperm pronuclei of Fucus distichus subsp. evanescens (C. Agardh) Powell was examined by fluorescence and electron microscopy when migration of the sperm pronucleus and, as a result, karyogamy were blocked by colchicine treatment after plasmogamy. Chromosome condensation. was observed in both pronuclei. Microspectrophotometric studies after staining the nuclei with mithramycin A clearly showed thatDNA synthesis occurred in the egg pronucleus but not in the sperm pronucleus. This means that chromosomes condensed prematurely in the sperm pronucleus (premature chromosome condensation). In some cases, the egg chromosomes became arranged on a metaphase plate, whereas the spermchromosomes lay scattered near the egg pronucleus. Immunofluorescencemicroscopy using anti-beta-tubulin antibody confirmed that a normal spindle was formed at the egg pronucleus. A pair of centrioles existed at the two poles of this spindle. The sperm nuclear membrane disappeared, and microtubules radiated to the sperm chromosomes from one pole of the egg spindle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:05:55