Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PRODUCTION OF A FUNCTIONAL ANTI-SCORPION HEMOCYANIN SCFV IN ESCHERICHIA-COLI
Autore:
BILLIALD P; MOTTA G; VAUX DJ;
Indirizzi:
UNIV OXFORD,SIR WILLIAM DUNN SCH PATHOL,S PARKS RD OXFORD OX1 3RE ENGLAND UNIV OXFORD,SIR WILLIAM DUNN SCH PATHOL OXFORD OX1 3RE ENGLAND UNIV ORLEANS,CNRS F-45071 ORLEANS 2 FRANCE
Titolo Testata:
Archives of biochemistry and biophysics
fascicolo: 2, volume: 317, anno: 1995,
pagine: 429 - 438
SICI:
0003-9861(1995)317:2<429:POAFAH>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SINGLE-CHAIN ANTIBODY; ANDROCTONUS-AUSTRALIS HEMOCYANIN; IMMUNOGLOBULIN VARIABLE DOMAINS; IMMUNOELECTRON MICROSCOPY; MONOCLONAL-ANTIBODY; FV FRAGMENT; INTRAMOLECULAR LOCALIZATION; FAB-FRAGMENTS; EXPRESSION; PROTEINS;
Keywords:
BACTERIAL EXPRESSION; IMMUNOCHEMISTRY; RECOMBINANT ANTIBODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
P. Billiald et al., "PRODUCTION OF A FUNCTIONAL ANTI-SCORPION HEMOCYANIN SCFV IN ESCHERICHIA-COLI", Archives of biochemistry and biophysics, 317(2), 1995, pp. 429-438

Abstract

A58 is a murine monoclonal antibody raised against the internal dimeric subunit Aa 1 of the hemocyanin of the scorpion Androctonus australis. When A58 is tested against a range of related hemocyanins, it exhibits cross-reactivity with a limited number of scorpion species. All ofthem belong to a single Scorpion Family: the Buthidae. We have utilized a bacterial expression-export system in which the variable regions of A58 are expressed as a single-chain variable fragment (scFv). Polymerase chain reaction (PCR) was used for the cloning and modification of the heavy and light variable regions which were assembled into a scFv. After insertion into a vector which contains the pectate lyase signal sequence from Erwinia caratovora for periplasmic expression, a scFvprotein was produced in high yield as a soluble and functional protein. The bacterial produced A58 scFv has binding properties similar to those of the original murine antibody. It binds specifically the heterodimeric subunit of Androctonus australis hemocyanin, cross-reacts withonly one subunit of each Buthidae investigated, and blocks the recognition of the hemocyanin by antibody A58 in competitive enzyme-linked immunosorbant assay. Previous reports have demonstrated the value of monoclonal antibodies in taxonomical and molecular evolution studies. A58scFv is the first of a new generation of antigen-binding proteins which should prove useful both in taxonomical studies and in the analysisof VH-VL structure-function relationships of antibodies. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 06:06:16