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Titolo:
IDENTIFICATION OF HERD-SPECIFIC BOVINE VIRAL DIARRHEA VIRUS ISOLATES FROM INFECTED CATTLE AND SHEEP
Autore:
PATON DJ; CARLSSON U; LOWINGS JP; SANDS JJ; VILCEK S; ALENIUS S;
Indirizzi:
CENT VET LAB WEYBRIDGE ADDLESTONE KT15 3NB SURREY ENGLAND SWEDISH UNIV AGR SCI,DEPT CATTLE & SHEEP DIS UPPSALA SWEDEN NATL VET INST S-75007 UPPSALA SWEDEN UNIV VET MED KOSICE,DEPT INFECTOL & TROP VET MED KOSICE SLOVAKIA
Titolo Testata:
Veterinary microbiology
fascicolo: 4, volume: 43, anno: 1995,
pagine: 283 - 294
SICI:
0378-1135(1995)43:4<283:IOHBVD>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOG-CHOLERA VIRUS; MONOCLONAL-ANTIBODIES; NUCLEOTIDE-SEQUENCE; MOLECULAR-CLONING; BORDER DISEASE; PESTIVIRUSES; PORCINE; STRAIN; REGION; PIGS;
Keywords:
BOVINE VIRAL DIARRHEA VIRUS; CATTLE, VIRUSES; SHEEP, VIRUSES; DIAGNOSIS, VIRUSES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
D.J. Paton et al., "IDENTIFICATION OF HERD-SPECIFIC BOVINE VIRAL DIARRHEA VIRUS ISOLATES FROM INFECTED CATTLE AND SHEEP", Veterinary microbiology, 43(4), 1995, pp. 283-294

Abstract

Thirteen pestiviruses isolated from ruminants on four different farmsin Sweden were compared antigenically and genetically. On two farms, viruses were isolated from both cattle and sheep, a third farm contained only sheep and a fourth only cattle. Seven viruses were isolated from six different cattle and six viruses were isolated from five different sheep. Epitope conservation between the viruses was studied with apanel of 32 monoclonal antibodies, revealing that all of the isolateswere BVDV-like. However, certain epitopes present in isolates from cattle were lost following virus transmission to sheep. In vitro amplification of the 5'-untranslated region of the 13 isolates by the polymerase chain reaction (PCR) and subsequent analyses of amplified productswith restriction enzymes also indicated that all 13 isolates belong to the BVDV group of pestiviruses. A fragment of the E2 (gp53) gene of each virus was amplified by PCR and a comparison of the amplified sequence of 188 nucleotides separated the isolates into four groups each of which could be identified with a particular farm of origin. The 13 isolates were thus herd-specific rather than species-specific demonstrating that BVDV is readily transmitted between cattle and sheep.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:05:34