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Titolo:
ASCORBATE FE3(+)-INDUCED PEROXIDATION AND INHIBITION OF THE BINDING OF A(1) ADENOSINE RECEPTOR LIGANDS IN RAT-BRAIN MEMBRANES/
Autore:
OLIVEIRA JC; CONSTANTINO MD; SEBASTIAO AM; RIBEIRO JA;
Indirizzi:
GULBENKIAN INST SCI,PHARMACOL LAB P-2781 OEIRAS PORTUGAL GULBENKIAN INST SCI,PHARMACOL LAB P-2781 OEIRAS PORTUGAL
Titolo Testata:
Neurochemistry international
fascicolo: 3, volume: 26, anno: 1995,
pagine: 263 - 268
SICI:
0197-0186(1995)26:3<263:AFPAIO>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED LIPID-PEROXIDATION; ALZHEIMERS-DISEASE; CORTICAL MEMBRANES; MOUSE-BRAIN; FLUIDITY; CORTEX; DEGENERATION; HIPPOCAMPUS; SEROTONIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
J.C. Oliveira et al., "ASCORBATE FE3(+)-INDUCED PEROXIDATION AND INHIBITION OF THE BINDING OF A(1) ADENOSINE RECEPTOR LIGANDS IN RAT-BRAIN MEMBRANES/", Neurochemistry international, 26(3), 1995, pp. 263-268

Abstract

The effect of peroxidation induced by the ascorbate/Fe3+ system on the binding properties of the A(1) adenosine receptor, was studied in rat brain membranes, using the agonist, [H-3]R-N-6-phenylisopropyladenosine ([H-3]R-PIA), and the antagonist, [H-3]1,3 -dipropyl-8-cyclopentylxanthine ([H-3]DPCPX). For the agonist, as well as for the antagonist,the number of binding sites (B-max) was significantly (P < 0.05) reduced after pretreatment of the membranes with ascorbate/Fe3+. The affinity of the agonist for the binding sites was not statistically modified (P > 0.05) after ascorbate/Fe3+ pretreatment, whereas the K-d value of the antagonist was increased (P < 0.05) by a factor of 2. Ascorbate/Fe3+ pretreatment affected agonist binding in the presence of GTP in a similar way as that observed in the absence of GTP, suggesting that peroxidation also affects agonist binding to A(1) adenosine receptors uncoupled to G-proteins. The results suggest that when brain membranessuffer free radical oxidative damage, the adenosine modulation of neuronal activity through A(1) receptors could be less efficient.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 06:56:43