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Titolo:
RAINBOW-TROUT ESTROGEN-RECEPTOR PRESENTS AN EQUAL SPECIFICITY BUT A DIFFERENTIAL SENSITIVITY FOR ESTROGENS THAN HUMAN ESTROGEN-RECEPTOR
Autore:
LEDREAN Y; KERN L; PAKDEL F; VALOTAIRE Y;
Indirizzi:
UNIV RENNES 1,INRA,BIOL MOLEC LAB,CNRS,URA 256,EQUIPE ENDOCRINOL MOLEC POISSONS,CAMPUS BEAULIEU F-35042 RENNES FRANCE UNIV RENNES 1,INRA,BIOL MOLEC LAB,CNRS,URA 256,EQUIPE ENDOCRINOL MOLEC POISSONS F-35042 RENNES FRANCE
Titolo Testata:
Molecular and cellular endocrinology
fascicolo: 1, volume: 109, anno: 1995,
pagine: 27 - 35
SICI:
0303-7207(1995)109:1<27:REPAES>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HORMONE-BINDING DOMAIN; TRANSCRIPTIONAL ACTIVATION; NUCLEAR TRANSLOCATION; TAMOXIFEN AZIRIDINE; STEROID-RECEPTORS; IDENTIFICATION; EXPRESSION; DNA; VITELLOGENIN; CELLS;
Keywords:
ESTRADIOL RECEPTOR; RAINBOW TROUT (ONCORHYNCHUS MYKISS); STEROIDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
Y. Ledrean et al., "RAINBOW-TROUT ESTROGEN-RECEPTOR PRESENTS AN EQUAL SPECIFICITY BUT A DIFFERENTIAL SENSITIVITY FOR ESTROGENS THAN HUMAN ESTROGEN-RECEPTOR", Molecular and cellular endocrinology, 109(1), 1995, pp. 27-35

Abstract

The estrogen receptor is a transcription factor that mediates the actions of estrogens which plays a major role in salmonid vitellogenesis. Previously, we cloned and described the estradiol receptor in rainbowtrout. To study the transactivation characteristics, the full length cDNA was inserted in an expression vector and tested by transfection on an estrogen-dependent promoter (pERE-TK-CAT), For the first time, direct comparison between the trout receptor (rtER) and human receptor (hER) in an analogous system has been possible. We demonstrate that rtER can, like hER, transactivate transcription in a strictly hormone-dependent manner. The specificity and sensitivity of the rtER response todifferent steroids have been studied. With rtER, the E2 concentrationneeded for half maximal activation is 10 times higher than with hER. In addition, we show that rtER has a weak affinity for androgens and transactivation could be induced using high testosterone concentration. Experiments show that both rtER and hER have an equal specificity forestrogens but that the rtER binds its ligand with a lower sensitivitythan hER. Several hypotheses concerning the role of main amino acids within the receptor protein are proposed based on the different properties of the receptors and comparison of sequences.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 13:47:17