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Titolo:
ANALYSIS OF THE LIPID-CONTENT AND THE MOTILITY OF HUMAN SPERM AFTER FOLLICULAR-FLUID TREATMENT
Autore:
HAMAMAH S; LANSON M; BARTHELEMY C; GARRIGUE MA; MUH JP; ROYERE D; LANSAC J;
Indirizzi:
CHU BRETONNEAU,DEPT OBSTET GYNECOL,UNITE BIOL REPROD F-37044 TOURS FRANCE CHU BRETONNEAU,INSERM,BIOL TUMERURS LAB F-37044 TOURS FRANCE CHU BRETONNEAU,INSERM,BIOCHIM LAB F-37044 TOURS FRANCE
Titolo Testata:
Andrologia
fascicolo: 2, volume: 27, anno: 1995,
pagine: 91 - 97
SICI:
0303-4569(1995)27:2<91:AOTLAT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACROSOME REACTION; HUMAN-SPERMATOZOA; HYPERACTIVATED MOTILITY; CAPACITATION; CHOLESTEROL; INVITRO; INDUCTION;
Keywords:
SPERMATOZOA; MOTILITY; LIPIDS; FOLLICULAR FLUID; HUMAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
S. Hamamah et al., "ANALYSIS OF THE LIPID-CONTENT AND THE MOTILITY OF HUMAN SPERM AFTER FOLLICULAR-FLUID TREATMENT", Andrologia, 27(2), 1995, pp. 91-97

Abstract

The effect of human follicular fluid (hFF) on the cholesterol and phospholipid content and the movement characteristics of human spermatozoa were studied. Semen was selected by a discontinuous Percoll gradientand incubated during in vitro capacitating conditions with B2 medium supplemented with hFF 20%. Percoll pelleted spermatozoa were incubatedin either B2 (B2-Percoll) or B2 supplemented with hFF (hFF-Percoll). In hFF-Percoll, we observed a time-dependent (24 h) decrease in both the cholesterol and phospholipid contents (cholesterol: 10.1 vs. 8.7 nmol 10(-7) spermatozoa; phospholipids: 17.5 vs. 15.7 nmol 10(-7) spermatozoa, P<0.05). This decrease in cholesterol and phospholipids in human spermatozoa was concomitant with a high straight line velocity, a high progressive motility percentage and an increased value of lateral head displacement without any significant alteration of the spermatozoal membrane. No modification of the cholesterol: phospholipid ratio after 2 and 24 h of incubation in either B2-Percoll (0.61, 0.54) in hFF-Percoll (0.59, 0.63) was observed when compared with original control semen. It is suggested that the decrease in cholesterol and phospholipids in hFF-Percoll may be taken into account for the changes of membrane modification as part of the capacitation process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 13:01:41