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Titolo:
CHARACTERIZATION OF THE GLYCOSYLATED ECDYSTEROIDS IN THE HEMOLYMPH OFBACULOVIRUS-INFECTED GYPSY-MOTH LARVAE AND CELLS IN CULTURE
Autore:
KELLY TJ; PARK EJ; MASLER CA; BURAND JP;
Indirizzi:
USDA ARS,INST PLANT SCI,INSECT NEUROBIOL & HORMONE LAB BELTSVILLE MD 20705
Titolo Testata:
European journal of entomology
fascicolo: 1, volume: 92, anno: 1995,
pagine: 51 - 61
SICI:
1210-5759(1995)92:1<51:COTGEI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Keywords:
BACULOVIRUS; LYMANTRIA DISPAR; NUCLEAR POLYHEDROSIS VIRUS; LDNPV; ECDYSONE; UDP-GLUCOSYL TRANSFERASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
NO
Recensione:
Indirizzi per estratti:
Citazione:
T.J. Kelly et al., "CHARACTERIZATION OF THE GLYCOSYLATED ECDYSTEROIDS IN THE HEMOLYMPH OFBACULOVIRUS-INFECTED GYPSY-MOTH LARVAE AND CELLS IN CULTURE", European journal of entomology, 92(1), 1995, pp. 51-61

Abstract

Fourth-instar gypsy moth (Lymantria dispar; Lepidoptera: Lymantriidae) larvae, infected with the gypsy moth baculovirus (LdNPV), show an elevated and prolonged extension of the hemolymph ecdysteroid titer peakassociated with molting. The ecdysteroid immunoreactivity associated with this peak elutes as two peaks following HPLC on a C-18 reverse-phase column. Both peaks elute in a region more polar than 20-hydroxyecdysone, but less polar than the highly polar ecdysteroid immunoreactivity associated with the apolysis peak of control animals. Glycosylated ecdysteroid standards, produced by in vitro incubation of UDP-glucose with ecdysone or 20-hydroxyecdysone and culture medium from LdNPV-infected gypsy moth cells, show elution times identical to the two immunoreactive peaks. Enzymatic hydrolysis studies verified this identity. The data suggest that the hemolymph of LdNPV-infected L. dispar larvae contains both glucose-conjugated ecdysone and glucose-conjugated 20-hydroxyecdysone which, by analogy with the literature, are presumably 22-O-beta-D-glucopyranoside and 20-hydroxyecdysone 22-O-beta-D-glucopyranoside.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 22:49:49