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Titolo:
MYOCARDIAL INOSITOLTRISPHOSPHATE IS DEPRESSED BY DIBUTYRYL-CAMP - AN EXPERIMENTAL-STUDY IN THE ISOLATED WORKING RAT-HEART
Autore:
MARTINUSSEN HJ; WALDENSTROM A; RONQUIST G;
Indirizzi:
UNIV UPPSALA HOSP,DEPT CARDIOL S-75185 UPPSALA SWEDEN UNIV UPPSALA HOSP,DEPT CARDIOL S-75185 UPPSALA SWEDEN UNIV UPPSALA HOSP,DEPT ANAESTHESIOL S-75185 UPPSALA SWEDEN UNIV UPPSALA HOSP,DEPT CLIN CHEM S-75185 UPPSALA SWEDEN
Titolo Testata:
Acta Physiologica Scandinavica
fascicolo: 2, volume: 153, anno: 1995,
pagine: 143 - 149
SICI:
0001-6772(1995)153:2<143:MIIDBD>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
INOSITOL TRISPHOSPHATE; PHOSPHATIDYLINOSITOL METABOLISM; ALPHA-ADRENOCEPTORS; BETA-ADRENOCEPTORS; CARDIAC-MUSCLE; STIMULATION; CONTRACTION; ALPHA-1-ADRENOCEPTORS; CARDIOMYOCYTES; CALCIUM;
Keywords:
ALPHA-RECEPTOR; BETA-RECEPTOR; CAMP; CGMP; DIBUTYRYL CAMP; INOSITOLTRISPHOSPHATE; ISOPROTERENOL; MECHANICAL RESPONSE; MYOCARDIUM; PHENYLEPHRINE; RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
H.J. Martinussen et al., "MYOCARDIAL INOSITOLTRISPHOSPHATE IS DEPRESSED BY DIBUTYRYL-CAMP - AN EXPERIMENTAL-STUDY IN THE ISOLATED WORKING RAT-HEART", Acta Physiologica Scandinavica, 153(2), 1995, pp. 143-149

Abstract

A possible interrelation between IP3 and cAMP was studied in rat myocardium through circumvention of the receptor mediated stimulatory stepof adenylyl cyclase by the administration of dibutyryl cAMP (db-cAMP). Changes in IP3 and cyclic nucleotide contents were correlated to changes in contractility after 40 min of beta- and alpha-adrenergic stimulation. Rat hearts (n = 23) were perfused with Krebs-Henseleit buffer in a modified Langendorff apparatus as a working preparation. The hearts were allocated to perfusion as control (n=6); or with phenylephrine(10(-6) mol L(-1), n=6); (-)-isoproterenol (10(-6) mol L(-1), n = 6);db-cAMP (2 x 10(-4) mol L(-1), n = 5). All hearts were freeze-clampedafter 40 min of perfusion. Phenylephrine produced a slow increase in (max)dP/dt reaching a maximal value after 10 min (P < 0.05); thereafter it decreased, reaching the control level at 30 min. Isoproterenol perfusion resulted in an early (20 s) increase in (max)dP/dt (P < 0.05). Over the next 10 s (max)dP/dt decreased markedly reaching an inflection point at 30 s. Thereafter only a slow increase during the rest of the perfusion was seen. Dibutyryl cAMP increased (max)dP/dt slowly during the whole perfusion period reaching maximum after 40 min. Cyclic-AMP was increased by 21% after 40 min of phenylephrine perfusion while the corresponding increases by isoproterenol and db-cAMP were 131 and 105%, respectively (P < 0.05). Phenylephrine increased IP3 content to the same extent as isoproterenol perfusion (P < 0.05). On the other hand, a decreased IP3 content was seen at 40 min of db-cAMP perfusion. Cyclic-AMP per se depressed basal myocardial IP3 content in myocardial tissue. Despite normalization of contractility after 40 min of phenylephrine perfusion the IP3 content was 27% higher than in control hearts pointing out the unclearness of the relationship between myocardial IP3 content and the contractile system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 20:04:56