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Titolo:
OVEREXPRESSION OF MITOCHONDRIAL GENES IN ALLOPLASMIC COMMON WHEAT WITH A CYTOPLASM OF WHEATGRASS (AGROPYRON TRICHOPHORUM) SHOWING DEPRESSEDVIGOR AND MALE-STERILITY
Autore:
SUZUKI T; NAKAMURA C; MORI N; KANEDA C;
Indirizzi:
KOBE UNIV,GRAD SCH SCI & TECHNOL,DIV BIOL RESOURCES,NADA KU KOBE 657 JAPAN KOBE UNIV,GRAD SCH SCI & TECHNOL,DIV BIOL RESOURCES,NADA KU KOBE 657 JAPAN KOBE UNIV,FAC AGR,GENET LAB,NADA KU KOBE 657 JAPAN
Titolo Testata:
Plant molecular biology
fascicolo: 3, volume: 27, anno: 1995,
pagine: 553 - 565
SICI:
0167-4412(1995)27:3<553:OOMGIA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-SEQUENCE; MAIZE MITOCHONDRIA; CYTOCHROME-OXIDASE; TRANSCRIPTION; FERTILITY; GENOME; GROWTH; LINES; DNA; CHLOROPLAST;
Keywords:
ALLOPLASMIC COMMON WHEAT; MITOCHONDRIAL GENE EXPRESSION; NUCLEUS-CYTOPLASM COMPATIBILITY/INCOMPATIBILITY; RUN-ON TRANSCRIPTION; STEADY-STATE TRANSCRIPT ABUNDANCE; VIGOR/FERTILITY-RESTORING CHROMOSOME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
T. Suzuki et al., "OVEREXPRESSION OF MITOCHONDRIAL GENES IN ALLOPLASMIC COMMON WHEAT WITH A CYTOPLASM OF WHEATGRASS (AGROPYRON TRICHOPHORUM) SHOWING DEPRESSEDVIGOR AND MALE-STERILITY", Plant molecular biology, 27(3), 1995, pp. 553-565

Abstract

An alloplasmic hybrid (nucleus-cytoplasm hybrid) of common wheat (Triticum aestivum) with a cytoplasm of wheatgrass (Agropyron trichophorurz) shows highly depressed vigor and complete male sterility. The presence of one short-arm telocentric homeologous group 1 chromosome (telosome) of the cytoplasm donor, however, restores normal vigor and male fertility of the hybrid. To study role(s) of the telosome on vigor/fertility restoration, mitochondrial genome organization and gene expression were compared among seedlings of the alloplasmic line showing depressed vigor, the corresponding restored line having a pair of the telosomes, and a euplasmic nuclear donor as control. No differences were detected in the mitochondrial genome structure between the depressed line and the restored line. Northern blot analysis using ten mitochondrial genes as probes showed no differences in transcript size and number between the depressed and restored lines, although clear differences were found in size of the major transcripts of two genes (cob and orf25) between the alloplasmic lines and the euplasmic control. Steady-state transcript levels were higher in the depressed line than in the other lines for all the mitochondrial genes analyzed including rrn18&5 when the same amount of mitochondrial RNA was loaded. The amount of rrn18&5 transcript in the total cellular RNA, however, did not differ amongthe lines. Run-on transcription analysis demonstrated markedly elevated transcriptional activities of all the mitochondrial genes analyzed in the depressed line based on unit amount of mitochondrial DNA, RNA and protein. The presence of Agropyron telosomes apparently normalized the level of mitochondrial transcription. These observations suggest either direct or indirect association of the observed mitochondrial gene overexpression with the depressed vigor and male sterility of the alloplasmic hybrid.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 16:01:00