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Titolo:
EXTRUDED ERYTHROBLAST NUCLEI ARE BOUND AND PHAGOCYTOSED BY A NOVEL MACROPHAGE RECEPTOR
Autore:
QIU LB; DICKSON H; HAJIBAGHERI MAN; CROCKER PR;
Indirizzi:
JOHN RADCLIFFE HOSP,INST MOLEC MED,IMPERIAL CANC RES FUND LABS OXFORDOX3 9DU ENGLAND JOHN RADCLIFFE HOSP,INST MOLEC MED,IMPERIAL CANC RES FUND LABS OXFORDOX3 9DU ENGLAND
Titolo Testata:
Blood
fascicolo: 6, volume: 85, anno: 1995,
pagine: 1630 - 1639
SICI:
0006-4971(1995)85:6<1630:EENABA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC-CELL CLUSTERS; MONOCLONAL-ANTIBODY; MOUSE MACROPHAGE; BONE-MARROW; MEDIATED PHAGOCYTOSIS; VITRONECTIN RECEPTOR; STROMAL MACROPHAGES; TISSUE MACROPHAGES; HUMAN-MONOCYTES; HEMAGGLUTININ;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
L.B. Qiu et al., "EXTRUDED ERYTHROBLAST NUCLEI ARE BOUND AND PHAGOCYTOSED BY A NOVEL MACROPHAGE RECEPTOR", Blood, 85(6), 1995, pp. 1630-1639

Abstract

Resident bone marrow macrophages (RBMM) play an important role in clearance of nuclei extruded from late-stage erythroblasts (Eb). To investigate the nature of macrophage receptors involved in this process, extruded erythroblast nuclei (EEN) were purified by cultivation of erythroblasts with erythropoietin, followed by density gradient centrifugation. By electron microscopy, the majority of free nuclei had an intactplasma membrane. EEN bound avidly to RBMM in a divalent cation-independent manner at both 4 degrees C and 37 degrees C. The use of specificmonoclonal antibodies (MoAbs) and inhibitors showed that this adhesive interaction was not mediated by previously characterized macrophage receptors involved in recognition of either developing hematopoietic cells or apoptotic cells. The EEN receptor was expressed on resident macrophages isolated from murine bone marrow, spleen, lymph node, and peritoneal cavity, but was completely absent from alveolar macrophages. Despite high levels of EEN binding to RBMM, few were phagocytosed evenafter prolonged culture. Phorbol myristate acetate (PMA) was found tostimulate phagocytosis, suggesting that this is a regulated process. These findings indicate that EEN are recognized by a novel macrophage receptor and that recognition may be triggered during the membrane remodeling that accompanies enucleation. (C) 1995 by The American Societyof Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 10:56:14