Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
INVOLVEMENT OF THIOL-GROUPS IN THE IMPAIRMENT OF CARDIAC SARCOPLASMICRETICULAR PHOSPHOLIPASE-D ACTIVITY BY OXIDANTS
Autore:
DAI JA; MEIJ JTA; DHALLA V; PANAGIA V;
Indirizzi:
ST BONIFACE GEN HOSP,RES CTR,DIV CARDIOVASC SCI,351 TACHE AVE WINNIPEG MB R2H 2A6 CANADA ST BONIFACE GEN HOSP,RES CTR,DIV CARDIOVASC SCI WINNIPEG MB R2H 2A6 CANADA UNIV MANITOBA,FAC MED,DEPT PHYSIOL WINNIPEG MB R2H 2A6 CANADA UNIV MANITOBA,FAC MED,DEPT ANAT WINNIPEG MB R2H 2A6 CANADA
Titolo Testata:
Journal of lipid mediators and cell signalling
fascicolo: 2, volume: 11, anno: 1995,
pagine: 107 - 118
SICI:
0929-7855(1995)11:2<107:IOTITI>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXYGEN FREE-RADICALS; HYDROGEN-PEROXIDE GENERATION; SULFHYDRYL-GROUPS; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHORYLATION; DITHIOTHREITOL; MITOCHONDRIA; HYDROLYSIS; MYOCARDIUM;
Keywords:
PHOSPHOLIPASE D; THIOL; HYDROGEN PEROXIDE; HYPOCHLOROUS ACID; SARCOPLASMIC RETICULAR MEMBRANE; RAT HEART;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
J.A. Dai et al., "INVOLVEMENT OF THIOL-GROUPS IN THE IMPAIRMENT OF CARDIAC SARCOPLASMICRETICULAR PHOSPHOLIPASE-D ACTIVITY BY OXIDANTS", Journal of lipid mediators and cell signalling, 11(2), 1995, pp. 107-118

Abstract

Considerable phospholipase D (PLD) activity is localized in myocardial sarcoplasmic reticular (SR) membranes, where it may take part in theregulation of Ca2+ movements. In this study, we examined thiol group dependence as a possible regulatory mechanism for SR PLD. SR membranesisolated from rat heart were exposed to four types of thiol group modifiers, which all induced a decrease in SR PLD activity that was prevented by dithiothreitol. Furthermore, since abnormalities in thiol status and Ca2+ homeostasis are characteristic for the myocardial cell damage induced by oxidative stress, we also studied the effects of oxidants on the SR PLD activity. The enzyme was not affected by xanthine-xanthine oxidase, but was depressed by hydrogen peroxide and by hypochlorous acid. These inhibitory effects were prevented by catalase as well as by methionine and dithiothreitol, respectively. Furthermore, reduced glutathione protected against the hydrogen peroxide-induced depression, whereas oxidized glutathione inhibited SR PLD. The results indicate that SR PLD activity is inhibited by nonradical oxidants, hydrogen peroxide and hypochlorous acid, through reversible modification of associated thiol groups. Thus, the enzyme may be controlled by the glutathione redox status of the cardiac cell.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:22:00