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Titolo:
IN-VITRO PRESERVATION OF HIGO CHRYSANTHEMUM (DENDRANTHEMA X GRANDIFLORUM (RAMAT) KITAM)
Autore:
ROXAS NJL; TASHIRO Y; MIYAZAKI S; ISSHIKI S; TAKESHITA A;
Indirizzi:
SAGA UNIV,FAC AGR SAGA 840 JAPAN
Titolo Testata:
Engei Gakkai Zasshi
fascicolo: 4, volume: 63, anno: 1995,
pagine: 863 - 870
SICI:
0013-7626(1995)63:4<863:IPOHC(>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
SHOOT TIPS; CRYOPRESERVATION; STORAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
11
Recensione:
Indirizzi per estratti:
Citazione:
N.J.L. Roxas et al., "IN-VITRO PRESERVATION OF HIGO CHRYSANTHEMUM (DENDRANTHEMA X GRANDIFLORUM (RAMAT) KITAM)", Engei Gakkai Zasshi, 63(4), 1995, pp. 863-870

Abstract

In order to establish a method for the preservation of Higo chrysanthemum cultivars (Dendranthema x grandiflorum (Ramat. ) Kitam.), studies were conducted on the influences of the type of explant, temperature, light and other conditions on the survival and growth of cultures in vitro. Cultures at 5 degrees C, 15 degrees C, 25 degrees C and 25 degrees-15 degrees-5 degrees C had maximum rates of 100%, 60%, 53% and 100%of survival, respectively, for a period of 12 months. Cultures at 15 degrees C and 25 degrees C showed a comparatively active growth and advanced senescence or withering after 12 months of incubation. On the other hand, cultures at 5 degrees C and 25 degrees-15 degrees-5 degreesC showed growth retardation and delayed senescence which had maximum rates of 100% and 75% survival, respectively after 24 months. Dark condition increased the survival rate of cultures at 5 degrees C althoughthe plant height was comparatively smaller than that under light. Nodal segments showed higher survival rate than the apical buds, and the removal of the expanded leaf on the explant showed advantages upon incubation. One ml of MS medium without growth regulators in a 12 X 75 mmtest tube was enough to ensure the survival of a culture at 5 degreesC for a period of 24 months without transferring to a new medium or without supplementing the medium. Based on the above results, a practically safe, economical and useful system for the in vitro preservation of Higo chrysanthemum has been established. This is presently applied to the 21 cultivars.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 09:12:27