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Titolo:
CAT8, A NEW ZINC CLUSTER-ENCODING GENE NECESSARY FOR DEREPRESSION OF GLUCONEOGENIC ENZYMES IN THE YEAST SACCHAROMYCES-CEREVISIAE
Autore:
HEDGES D; PROFT M; ENTIAN KD;
Indirizzi:
UNIV FRANKFURT,BIOZENTRUM NIEDERURSEL,INST MIKROBIOL,MARIE CURIE STR 9 D-60439 FRANKFURT GERMANY UNIV FRANKFURT,BIOZENTRUM NIEDERURSEL,INST MIKROBIOL D-60439 FRANKFURT GERMANY
Titolo Testata:
Molecular and cellular biology
fascicolo: 4, volume: 15, anno: 1995,
pagine: 1915 - 1922
SICI:
0270-7306(1995)15:4<1915:CANZCG>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARBON CATABOLITE REPRESSION; GLUCOSE REPRESSION; ESCHERICHIA-COLI; SHUTTLE VECTORS; PROTEIN-KINASE; HEXOKINASE PII; MIG1 REPRESSOR; LACZ FUSIONS; MUTANTS; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
D. Hedges et al., "CAT8, A NEW ZINC CLUSTER-ENCODING GENE NECESSARY FOR DEREPRESSION OF GLUCONEOGENIC ENZYMES IN THE YEAST SACCHAROMYCES-CEREVISIAE", Molecular and cellular biology, 15(4), 1995, pp. 1915-1922

Abstract

The expression of gluconeogenic fructose-1,6-bisphosphatase (encoded by the FBP1 gene) depends on the carbon source. Analysis of the FBP1 promoter revealed two upstream activating elements, UASI(FBP1) AND UASZ(FBP1), which confer carbon source-dependent regulation on a heterologous reporter gene. On glucose media neither element was activated, whereas after transfer to ethanol a 100-fold derepression was observed. This gene activation depended on the previously identified derepressiongenes C;ATI (SNF1) (encoding a protein kinase) and CAT3 (SNF4) (probably encoding a subunit of Catlp [Snf1p]). Screening for mutations specifically involved in UBS1(FBP1) derepression revealed the new recessive derepression mutation cat8. The cat8 mutants also failed to derepress UAs2(FBP1) and these mutants were unable to grow on nonfermentable carbon sources. The CAT8 gene encodes a zinc cluster protein related toSacchnromgces cerevisiae Ga14p. Deletion of CAT8 caused a defect in glucose derepression which affected all keg gluconeogenic enzymes. Derepression of glucose-repressible invertase and maltase was still normally regulated. A CAT8-lacZ promoter fusion revealed that the CAT8 gene itself is repressed by Cat4p (Mig1p). These results suggest that gluconeogenic genes are derepressed upon binding of Cat8p, whose synthesis depends on the release of Cat lp (Mig1p) from the CAT8 promoter. However, gluconeogenic promoters are still glucose repressed in cat4 mutants, which indicates that in addition to its transcription, the Cat8p protein needs further activation. The observation that multicopy expression of CAT8 reverses the inability of cat1 and cat3 mutants to grow onethanol indicates that Cat8p might be the substrate of the Cat1p/Ca3pprotein kinase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 15:37:51