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Titolo:
CAUSES AND LOCATION OF NONSPECIFIC EFFECTS OF SHAM ON O-2 UPTAKE BY WHEAT ROOTS
Autore:
BINGHAM IJ; STEVENSON EA;
Indirizzi:
SAC ABERDEEN,DEPT CROP BIOL,581 KING ST ABERDEEN AB9 1UD SCOTLAND
Titolo Testata:
Physiologia Plantarum
fascicolo: 3, volume: 93, anno: 1995,
pagine: 427 - 434
SICI:
0031-9317(1995)93:3<427:CALONE>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARMORACIA-LAPATHIFOLIA-GILIB; HYDROGEN-PEROXIDE FORMATION; ISOLATED CELL-WALLS; ELECTRON FLUX; RESPIRATION; MITOCHONDRIA; HYDROXAMATE; CONSEQUENCES; CYTOCHROME; PATHWAY;
Keywords:
ALTERNATIVE PATHWAY; CYANIDE; CYTOCHROME PATHWAY; NONSPECIFICITY; O-2; PEROXIDASE; RESPIRATION; ROOT; SHAM; TRITICUM AESTIVUM; WHEAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
I.J. Bingham e E.A. Stevenson, "CAUSES AND LOCATION OF NONSPECIFIC EFFECTS OF SHAM ON O-2 UPTAKE BY WHEAT ROOTS", Physiologia Plantarum, 93(3), 1995, pp. 427-434

Abstract

Uptake of O-2 by whole, detached, root systems of wheal (Triticum aestivum L. cv. Alexandria) was titrated with salicylhydroxamic acid (SHAM) in the presence and absence of cyanide. The resulting rho(alt) plotwas non-linear indicating that SHAM was acting non-specifically. The nature of the non-specific effects was investigated in reverse titration experiments. Uptake of O-2 was titrated with KCN in the presence and absence of SHAM at 1 mM and 25 mM, which yielded rho(cyt) values of <1 and <1, respectively. The results suggest that at 25 mM, SHAM inhibits the cytochrome pathway, but at 1 mM it stimulates an O-2-consumingprocess which is likely to be a peroxidase. A SHAM-stimulated peroxidase could easily be washed from these roots. In vitro, the peroxidase was stimulated to a similar extent by low (1 mM) and high (25 mM) concentrations of SHAM. Failure to inhibit with high concentrations of SHAM distinguishes this peroxidase from those hitherto eluted from root tissue. Reverse titration experiments in the presence and absence of 1 mM SHAM indicated that there were no significant side effects of SHAM in root tips. These data are supported by the negligible peroxidase activity that was washed from this root fraction. In contrast, significant side effects occurred in vivo, and substantial peroxidase activity was measured in vitro, from sections 4-6 cm and 18-20 cm behind the seminal root apex. The greatest activity was found with the 4-6 cm section which may be associated with high rates of cell wall lignification. The implications of these results for measurements of root respiration are discussed.

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Documento generato il 30/11/20 alle ore 16:21:45