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Titolo:
SEPARATION OF EQUINE IGG SUBCLASSES (IGGA, IGGB AND IGG(T)) USING THEIR DIFFERENTIAL BINDING CHARACTERISTICS FOR STAPHYLOCOCCAL PROTEIN-A AND STREPTOCOCCAL PROTEIN-G
Autore:
SHEORAN AS; HOLMES MA;
Indirizzi:
UNIV CAMBRIDGE,DEPT CLIN VET MED,MADINGLEY RD CAMBRIDGE CB3 0ES ENGLAND UNIV CAMBRIDGE,DEPT CLIN VET MED CAMBRIDGE CB3 0ES ENGLAND
Titolo Testata:
Veterinary immunology and immunopathology
fascicolo: 1-3, volume: 55, anno: 1996,
pagine: 33 - 43
SICI:
0165-2427(1996)55:1-3<33:SOEIS(>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
FC-RECEPTORS;
Keywords:
EQUINE; IMMUNOGLOBULIN; PROTEIN A; PROTEIN G; IGG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
A.S. Sheoran e M.A. Holmes, "SEPARATION OF EQUINE IGG SUBCLASSES (IGGA, IGGB AND IGG(T)) USING THEIR DIFFERENTIAL BINDING CHARACTERISTICS FOR STAPHYLOCOCCAL PROTEIN-A AND STREPTOCOCCAL PROTEIN-G", Veterinary immunology and immunopathology, 55(1-3), 1996, pp. 33-43

Abstract

Equine IgG possesses four well-defined subisotypes, designated IgGa, IgGb, IgGc and IgG(T) on the basis of their increasing anodal mobilityin electrophoresis. However, the preparation of IgGa and IgGb reference proteins has not previously been reported. Certain bacterial cell wall proteins, termed protein A and protein G, have been used for purification of IgG subisotypes from the serum of domestic animals which, combined with other techniques utilising differences in the physico-chemical properties of the proteins, has allowed the purification of Ig isotypes. This paper describes purification of the subisotypes of equine IgG. Purification of IgGa and IgGb was achieved by the separation ofa 'fall-through' peak from ion-exchange chromatography consisting of IgGa and IgGb into two fractions (peaks C and D) by FPLC protein A andprotein G affinity chromatography. Peak C consisted of IgGb and peak D consisted of IgGa exhibiting slightly faster cathodal migration thanpeak C in IEP analysis. Affinity chromatography using protein A and Gcolumns also indicated that there may be two different components of IgG(T); one with a low affinity for protein G and the other having a greater affinity for protein G.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 06:43:25