Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
VIRAL RESPIRATORY-INFECTION INCREASES ALVEOLAR MACROPHAGE CYTOPLASMICMOTILITY IN RATS - ROLE OF NO
Autore:
FUKUSHIMA T; SEKIZAWA K; YAMAYA M; OKINAGA S; SATOH M; SASAKI H;
Indirizzi:
TOHOKU UNIV,SCH MED,DEPT GERIATR MED,AOBA KU,SEIRYO MACHI 1-1 SENDAI MIYAGI 980 JAPAN TOHOKU UNIV,SCH MED,DEPT GERIATR MED,AOBA KU SENDAI MIYAGI 980 JAPAN EISAI CO,DEPT MICROBIOL TOKYO 11288 JAPAN
Titolo Testata:
American journal of physiology. Lung cellular and molecular physiology
fascicolo: 3, volume: 12, anno: 1995,
pagine: 399 - 406
SICI:
1040-0605(1995)12:3<399:VRIAMC>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHESIS; SENDAI VIRUS; PULMONARY MACROPHAGES; L-ARGININE; INHIBITION; PARTICLES; PROTEIN;
Keywords:
VIRAL PNEUMONIA; GUANYLATE CYCLASE; HOST-DEFENSE MECHANISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
T. Fukushima et al., "VIRAL RESPIRATORY-INFECTION INCREASES ALVEOLAR MACROPHAGE CYTOPLASMICMOTILITY IN RATS - ROLE OF NO", American journal of physiology. Lung cellular and molecular physiology, 12(3), 1995, pp. 399-406

Abstract

Ingested ferrimagnetic (Fe3O4) particles were used to estimate noninvasively the motion of organelles in alveolar macrophages (AM) in intact rats during viral respiratory infection by parainfluenza type 1 (Sendai) virus. Four days after instillation of Fe3O4 particles (3 mg/kg) into the lung, remnant field strength (RFS) was measured at the body surface immediately after magnetization of Fe3O4 particles by an externally applied magnetic field. RFS decreases with time, due to particle rotation (relaxation) which is related to cytoplasmic motility of AM. Viral infection increased the relaxation rate (lambda(0) per min), andincreases in lambda(0) reached a maximum 3 days after nasal inoculation (day 3). Viral infection (day 3)-induced increases in lambda(0) were dose dependently inhibited by either the L-arginine analogue N-nitro-L-arginine or by methylene blue, an inhibitor of guanylate cyclase activity. Bronchoalveolar lavage fluid obtained from infected rats contained significantly higher levels of nitrite than that from control rats (P < 0.01). In in vitro experiments, AM from infected rats showed significantly higher lambda(0), nitrite production, and intracellular guanosine 3',5'-cyclic monophosphate levels than those from control rats(P < 0.01). Sodium nitroprusside, known to release nitric oxide concentration dependently, increased lambda(0) of AM from noninfected rats in vitro. These results suggest that nitric oxide plays an important role in AM cytoplasmic motility during viral respiratory infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 06:44:19