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Titolo:
DEMONSTRATION OF P53 PROTEIN AND TP53 GENE-MUTATIONS IN OLIGODENDROGLIOMAS
Autore:
HAGEL C; LAKING G; LAAS R; SCHEIL S; JUNG R; MILDELANGOSCH K; STAVROU DK;
Indirizzi:
UNIV HAMBURG,DEPT NEUROPATHOL,MARTINISTR 52 D-20246 HAMBURG GERMANY UNIV HAMBURG,DEPT CLIN CHEM D-20246 HAMBURG GERMANY UNIV HAMBURG,DEPT GYNECOOPATHOL D-20246 HAMBURG GERMANY
Titolo Testata:
European journal of cancer
fascicolo: 13, volume: 32A, anno: 1996,
pagine: 2242 - 2248
SICI:
0959-8049(1996)32A:13<2242:DOPPAT>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; TUMOR-SUPPRESSOR GENE; HUMAN BRAIN-TUMORS; STRAND CONFORMATION POLYMORPHISM; ASTROCYTIC TUMORS; GLIOBLASTOMA-MULTIFORME; CANCER;
Keywords:
OLIGODENDROGLIOMAS; P53 PROTEIN ACCUMULATION; TP53 GENE MUTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
C. Hagel et al., "DEMONSTRATION OF P53 PROTEIN AND TP53 GENE-MUTATIONS IN OLIGODENDROGLIOMAS", European journal of cancer, 32A(13), 1996, pp. 2242-2248

Abstract

Paraffin embedded tissue of 84 oligodendrogliomas (63 primary tumours, 21 recurrences), 21 glioblastomas with oligodendroglial growth pattern (15 primaries, 6 recurrences) and 17 mixed gliomas was investigatedfor the presence of mutations in exons 5-9 by means of single stranded conformation polymorphism (SCCP), temperature gradient gel electrophoresis (TGGE) and direct DNA sequencing. In parallel, p53 protein accumulation was determined by means of immunohistochemistry. The percentage of mutations was found to be higher than previously reported (6 of 44 grade II oligodendrogliomas, 4 of 19 grade III oligodendrogliomas, 4 of 15 glioblastomas). In 4 cases, the mutations lead to distinct changes in the primary or secondary structure of the protein (cysteine -->,tyrosine, proline --> leucine) and were associated with marked accumulation of p53 protein. A significant correlation between p53 protein accumulation and TP53 gene aberrations was found (P < 0.001), althoughp53 protein accumulation was detected more often than TP53 gene anomalies, indicating that factors other than TP53 gene mutation may also lead to a p53 protein accumulation in the tumour cells. A significant correlation was found for p53 protein accumulation and tumour grade butnot TP53 gene mutations. In conclusion, evaluation of p53 protein accumulation reflected the clinical course of oligodendrogliomas better than the mere presence of TP53 gene mutations. Copyright (C) 1996 Elsevier Science Ltd

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/21 alle ore 14:34:11