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Titolo:
SUBMERGED BATCH CULTURE OF THE PSYCHROPHILE MONOGRAPHELLA-NIVALIS IN A DEFINED MEDIUM - GROWTH, CARBOHYDRATE UTILIZATION AND RESPONSES TO TEMPERATURE
Autore:
CAIRNS AJ; HOWARTH CJ; POLLOCK CJ;
Indirizzi:
AFRC,INST GRASSLAND & ENVIRONM RES,DEPT CELL BIOL,PLAS GOGERDDAN ABERYSTWYTH SY23 3EB DYFED WALES AFRC,INST GRASSLAND & ENVIRONM RES,DEPT ENVIRONM BIOL ABERYSTWYTH SY23 3EB DYFED WALES
Titolo Testata:
New phytologist
fascicolo: 2, volume: 129, anno: 1995,
pagine: 299 - 308
SICI:
0028-646X(1995)129:2<299:SBCOTP>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
FRUCTOSYL TRANSFERASE-ACTIVITY; LOLIUM-TEMULENTUM L; HEAT-SHOCK PROTEINS; SACCHAROMYCES-CEREVISIAE; FRUCTAN BIOSYNTHESIS; EXCISED LEAVES; GLUCOFRUCTOSAN BIOSYNTHESIS; ASPERGILLUS-NIDULANS; FUSARIUM-OXYSPORUM; GENE-EXPRESSION;
Keywords:
CRYOPROTECTANT; FRUCTAN; HEAT SHOCK; INVERTASE; SNOW MOLD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
A.J. Cairns et al., "SUBMERGED BATCH CULTURE OF THE PSYCHROPHILE MONOGRAPHELLA-NIVALIS IN A DEFINED MEDIUM - GROWTH, CARBOHYDRATE UTILIZATION AND RESPONSES TO TEMPERATURE", New phytologist, 129(2), 1995, pp. 299-308

Abstract

An asporogenous strain of the pink snow mould fungus, Monographella nivalis (Schaffnit) E. Muller, anamorph Gerlachia nivalis (Ces. ex Sac. ) W. Gams and E. Muller (Syn. Fusarium nivale Ces. ex Sacc.), grew at 5 degrees C on a defined salts medium plus vitamins and utilized a variety of simple and polymeric carbohydrates as the sole carbon and energy source. Mycelium was grown at temperatures between 3 and 15 degreesC in aerated submerged fermentation culture in chemically defined medium plus sucrose. Optimum growth rates of 0.035-0.033 h(-1) occurred between 9 and 12 degrees C. Growth in a simple medium showed that all biochemical and physiological processes necessary for growth were functional at 3 degrees C. The growth performance of the organism at low temperatures was no better than would be expected from extrapolation of mesophilic growth responses to temperature. The optimum growth temperature of 9-12 degrees C showed that some biochemical or physiological process was impaired above 12 degrees C. Uptake and incorporation of S-35-methionine by mycelium at different temperatures showed that general protein synthesis increased up to 25 degrees C, and hence was not responsible per se for the sensitivity-to temperatures above 12 degrees C. Heat shock proteins were synthesized at the relatively low temperature of 25 degrees C, consistent with the low temperature optimum for growth. When grown with sucrose as the sole carbon source, the myceliumcatalyzed the extracellular hydrolysis of sucrose, releasing glucose and fructose together with a small amount of fructan trisaccharides and a trace of tetra- and penta-saccharides. Fructan accumulation was transient, corresponding with maximal rates of sucrose hydrolysis. Most biomass formation occurred in the absence of fructan in the culture, hence fructan was not necessary for growth at low temperature and did not appear to function as a cryoprotectant. Invertase activity was mostly (60-70%) bound to mycelium; the remainder was free in the culture supernatant. The regulation of invertase expression appeared to be by sucrose-induction, rather than by end-product repression. Rates of sucrose hydrolysis in culture were temperature-sensitive and were markedlydepressed above 12 degrees C, indicating inhibition of invertase formation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 20:11:43