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Titolo:
CYTOMEGALOVIRUS-MEDIATED INDUCTION OF ANTISENSE MESSENGER-RNA EXPRESSION TO UL44 INHIBITS VIRUS-REPLICATION IN AN ASTROCYTOMA CELL FINE - IDENTIFICATION OF AN ESSENTIAL GENE
Autore:
RIPALTI A; BOCCUNI MC; CAMPANINI F; LANDINI MP;
Indirizzi:
POLICLIN S ORSOLA,FAC MED & CHIRURG,IST MICROBIOL,VIA MASSARENTI 9 I-40138 BOLOGNA ITALY
Titolo Testata:
Journal of virology
fascicolo: 4, volume: 69, anno: 1995,
pagine: 2047 - 2057
SICI:
0022-538X(1995)69:4<2047:CIOAME>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT DNA-SYNTHESIS; IMMEDIATE-EARLY GENE; HUMAN T-CELLS; RNA EXPRESSION; TYPE-1 REPLICATION; FUSION PROTEINS; SENSE RNA; REGION; ACTIVATION; ENHANCER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
A. Ripalti et al., "CYTOMEGALOVIRUS-MEDIATED INDUCTION OF ANTISENSE MESSENGER-RNA EXPRESSION TO UL44 INHIBITS VIRUS-REPLICATION IN AN ASTROCYTOMA CELL FINE - IDENTIFICATION OF AN ESSENTIAL GENE", Journal of virology, 69(4), 1995, pp. 2047-2057

Abstract

We have used an antisense RNA approach in the analysis of gene function in human cytomegalovirus (HCMV). An astrocytoma cell line (U373-MG)that is permissive for virus replication was permanently transfected with a construct bearing sequence from HCMV UL44 (coding for the majorlate DNA-binding protein, ppUL44, also known as pp52 or ICP36) in an antisense orientation and under the control of the immediate-early enhancer-promoter element. Upon HCMV infection at a high multiplicity, wefound a marked reduction in UL44 protein products (the ICP36 family of proteins) in established cell transfectants and a strong inhibition of virus yield in infected-cell supernatants at two weeks postinfection, while herpes simplex virus replication was not affected. In infected cells, viral DNA replication was strongly inhibited. While gene products such as pUS22 and pUL32 were also inhibited, pUL123 and pUL82 accumulated in the infected cells over time. Our data suggest an essential role for the UL44 family of proteins in HCMV replication and represent a model of virus inhibition by virus-induced antisense RNA synthesis in genetically modified cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 07:20:05