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Titolo:
2 KINETICALLY DISTINCT 5-HYDROXYTRYPTAMINE-ACTIVATED CL- CONDUCTANCESAT RETZIUS P-CELL SYNAPSES OF THE MEDICINAL LEECH
Autore:
LESSMANN V; DIETZEL ID;
Indirizzi:
RUHR UNIV BOCHUM,LEHRSTUHL MOLEK NEUROBIOCHEM,GEBAUDE NC7-170,UNIV STR 150 W-4630 BOCHUM GERMANY RUHR UNIV BOCHUM,LEHRSTUHL MOLEK NEUROBIOCHEM W-4630 BOCHUM GERMANY
Titolo Testata:
The Journal of neuroscience
fascicolo: 2, volume: 15, anno: 1995,
pagine: 1496 - 1505
SICI:
0270-6474(1995)15:2<1496:2KD5CC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTAMATE-RECEPTOR DESENSITIZATION; EXCITATORY POSTSYNAPTIC CURRENTS; RAT HIPPOCAMPAL-NEURONS; AMPA KAINATE RECEPTORS; CENTRAL-NERVOUS-SYSTEM; SYNAPTIC CURRENTS; ACETYLCHOLINE-RECEPTOR; HIRUDO-MEDICINALIS; ACTIVATED CHANNELS; SENSORY NEURONS;
Keywords:
MEDICINAL LEECH; 5-HT; CL- CURRENTS; DESENSITIZATION; SEROTONERGIC SYNAPTIC TRANSMISSION; RETZIUS P-CELL SYNAPSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
V. Lessmann e I.D. Dietzel, "2 KINETICALLY DISTINCT 5-HYDROXYTRYPTAMINE-ACTIVATED CL- CONDUCTANCESAT RETZIUS P-CELL SYNAPSES OF THE MEDICINAL LEECH", The Journal of neuroscience, 15(2), 1995, pp. 1496-1505

Abstract

The properties of serotonin (5-HT)-activated Cl- receptor/ion channelcomplexes in neurons of the CNS of the medicinal leech were analyzed. These channels mediate the postsynaptic response at the serotoninergic Retzius P-cell as well as Retzius-Retzius cell synapses. 5-HT-induced Cl- currents were activated by fast superfusion of transmitter on cells dissociated from embryonic leeches including histochemically identified Retzius cells. Whole-cell currents elicited by partial superfusion of the membrane and summated single-channel currents of outside-outpatches showed times to peak of 18 +/- 4 msec and 10 +/- 5 msec, respectively, and desensitized with time constants of 28 +/- 3 msec and 20+/- 11 msec. Persistence of single-channel openings in the outside-out configuration as well as lack of effect of dialysis of the whole cell with AMP-PNP or GDP-beta-S indicated that 5-HT directly gates the Cl- channels without involving second messenger cascades. In outside-outpatches, two single-channel conductances of 13 pS and 32 pS were identified. While the 13 pS conductance desensitized, the 32 pS conductance activated within several tens of msec and showed no desensitization,We postulate that two subtypes of channels are coactivated by 5-HT and that the activation of the fast desensitizing channel could be responsible for the fast decaying component of the postsynaptic response. The slow conductance explains the second slower decay time constant of the postsynaptic response and could account for the tonic component sometimes observed at Retzius P-cell synapses.

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Documento generato il 13/07/20 alle ore 16:25:41