Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MOLECULAR ANALYSIS OF THE REGULATION OF MUSCARINIC RECEPTOR EXPRESSION AND FUNCTION
Autore:
HAMILTON SE; MCKINNON LA; JACKSON DA; GOLDMAN PS; MIGEON JC; HABECKER BA; THOMAS SL; NATHANSON NM;
Indirizzi:
UNIV WASHINGTON,DEPT PHARMACOL,SJ-30 SEATTLE WA 98195
Titolo Testata:
Life sciences
fascicolo: 11-12, volume: 56, anno: 1995,
pagine: 939 - 943
SICI:
0024-3205(1995)56:11-12<939:MAOTRO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA EXPRESSION;
Keywords:
MESSENGER-RNA; G-PROTEIN; MUSCARINIC RECEPTOR; GENE DISRUPTION; TRANSFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
10
Recensione:
Indirizzi per estratti:
Citazione:
S.E. Hamilton et al., "MOLECULAR ANALYSIS OF THE REGULATION OF MUSCARINIC RECEPTOR EXPRESSION AND FUNCTION", Life sciences, 56(11-12), 1995, pp. 939-943

Abstract

Several systems are being used to determine the molecular and cellular basis for the regulation of expression and function of the muscarinic receptors. Treatment of chick heart cells in culture results in decreased levels of mRNA encoding the cm2 and cm4 receptors. This probablyresults from decreased gene transcription which requires concomitant mAChR-mediated inhibition of adenylyl cyclase and mAChR-mediated stimulation of phospholipase C. Site-directed mutagenesis was used to demonstrate that the single tyrosine residue in the carboxyl-terminal cytoplasmic tail of the m2 receptor is involved in agonist-induced downregulation but not sequestration. Activation of heterologous receptors in chick heart cells can also regulate mAChR mRNA levels. A cAMP-regulated luciferase reporter gene has been used to demonstrate that the m4 receptor preferentially couples to G(i) alpha-2 or G(o) alpha over G(i alpha)-1 or G(i) alpha-3 to mediate inhibition of adenylyl cyclase activity. Finally, in order to determine the role of individual receptor subtypes in muscarinic-mediated responses in vivo, we are beginning to use the method of targeted gene disruption by homologous recombinationto generate mice deficient in specific receptor subtypes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 20:14:05