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Titolo:
ANALYSIS OF THE 2-KILOBASE LATENCY-ASSOCIATED TRANSCRIPT EXPRESSED INPC12 CELLS PRODUCTIVELY INFECTED WITH HERPES-SIMPLEX VIRUS TYPE-1 - EVIDENCE FOR A STABLE, NONLINEAR STRUCTURE
Autore:
RODAHL E; HAARR L;
Indirizzi:
UNIV BERGEN,CTR RES VIROL N-5020 BERGEN NORWAY UNIV BERGEN,CTR RES VIROL N-5020 BERGEN NORWAY
Titolo Testata:
Journal of virology
fascicolo: 2, volume: 71, anno: 1997,
pagine: 1703 - 1707
SICI:
0022-538X(1997)71:2<1703:AOT2LT>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRE-MESSENGER-RNA; NERVE GROWTH-FACTOR; DEBRANCHING ENZYME; TRIGEMINAL GANGLIA; SPLICING INVITRO; RIBOSOMAL-RNA; LARIAT; INTRON; PROMOTER; NEURONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
E. Rodahl e L. Haarr, "ANALYSIS OF THE 2-KILOBASE LATENCY-ASSOCIATED TRANSCRIPT EXPRESSED INPC12 CELLS PRODUCTIVELY INFECTED WITH HERPES-SIMPLEX VIRUS TYPE-1 - EVIDENCE FOR A STABLE, NONLINEAR STRUCTURE", Journal of virology, 71(2), 1997, pp. 1703-1707

Abstract

The major latency-associated transcript (LAT) expressed in PC12 cellsproductively infected with herpes simplex virus type 1 is a 2-kb, nonpolyadenylated RNA molecule that accumulates in the nuclei of infectedcells. In actinomycin D-treated cells, the 2 kb WT gene transcript has a half-life considerably greater than 12 h. After polyacrylamide gelelectrophoresis, two species of the transcript were observed, a majorspecies that was retarded in the gel and a minor species that migrated as a 1.96-kb RNA molecule, RNase H digestion after hybridization of the RNA with an oligonucleotide complementary to positions -80 to -101relative to the 3' end of the 2-kb LAT gene transcript changed the mobility of the retarded species into that of the rapidly migrating species. Our data indicate that the 2-kb LAT gene transcript expressed in productively infected PC12 cells is present in a stable, nonlinear form.

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Documento generato il 05/12/20 alle ore 01:32:33