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Titolo:
VARIATIONS IN TRANSCRIPTION-REPAIR COUPLING IN MOUSE CELLS
Autore:
MURAD AO; DECOCK J; BROWN D; SMERDON MJ;
Indirizzi:
WASHINGTON STATE UNIV,DEPT BIOCHEM & BIOPHYS PULLMAN WA 99164 WASHINGTON STATE UNIV,DEPT BIOCHEM & BIOPHYS PULLMAN WA 99164
Titolo Testata:
The Journal of biological chemistry
fascicolo: 8, volume: 270, anno: 1995,
pagine: 3949 - 3957
SICI:
0021-9258(1995)270:8<3949:VITCIM>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLOBUTANE PYRIMIDINE DIMERS; STRAND-SPECIFIC REPAIR; TUMOR VIRUS PROMOTER; NUCLEOSOME CORE DNA; GLUCOCORTICOID RECEPTOR; YEAST MINICHROMOSOME; ACTIVE GENE; GROUP-C; SEQUENCE; CHROMATIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
A.O. Murad et al., "VARIATIONS IN TRANSCRIPTION-REPAIR COUPLING IN MOUSE CELLS", The Journal of biological chemistry, 270(8), 1995, pp. 3949-3957

Abstract

Formation and repair of W-induced cyclobutane pyrimidine dimers (CPDs) was examined in three different genes in mouse L cells: 1) a stably integrated insert (called LTL), consisting of a herpes simplex virus thymidine kinase gene (th) fused to a hormone inducible promotor (LTR);2) the constitutively expressed protooncogene c-abl; and 3) the inactive immunoglobulin J chain gene, Transcription of the tk gene is induced >50-fold by dexamethasone. There is a nonuniform distribution of CPDs in LTL DNA irradiated in vitro, being 4-fold higher in the LTR thanin the th gene, indicating the LTR may be damaged preferentially in irradiated cells, Repair of CPDs occurs efficiently in both strands of LTL and is unaffected by hormone induction of th gene transcription. Transcription of tit mRNA is very sensitive to UV damage and follows single hit kinetics with UV dose. Furthermore, tit mRNA expression rapidly recovers during repair incubation, Transcription-coupled repair occurs in these cells, however, since only the transcribed strand of c-abl is efficiently repaired of CPDs; the nontranscribed strand as well as both strands of the J chain gene are inefficiently repaired. Thus, repair in the LTL construct may reflect a lack of transcription-coupledrepair in either the LTR promotor or the LTL insertion region of chromatin.

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Documento generato il 04/04/20 alle ore 08:39:37