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Titolo:
A DNA-POLYMERASE-BETA IN THE MITOCHONDRION OF THE TRYPANOSOMATID CRITHIDIA-FASCICULATA
Autore:
TORRI AF; ENGLUND PT;
Indirizzi:
JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM BALTIMORE MD 21205
Titolo Testata:
The Journal of biological chemistry
fascicolo: 8, volume: 270, anno: 1995,
pagine: 3495 - 3497
SICI:
0021-9258(1995)270:8<3495:ADITMO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
KINETOPLAST DNA; REPLICATION; MINICIRCLES; NETWORKS; TOPOISOMERASE; COMPILATION; ALIGNMENT; SITES;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
A.F. Torri e P.T. Englund, "A DNA-POLYMERASE-BETA IN THE MITOCHONDRION OF THE TRYPANOSOMATID CRITHIDIA-FASCICULATA", The Journal of biological chemistry, 270(8), 1995, pp. 3495-3497

Abstract

We previously purified a Crithidia fasciculata mitochondrial DNA polymerase that has unusual properties. Unlike a conventional mitochondrial DNA polymerase gamma, this enzyme is small, non-processive, deficient in 3'-exonuclease activity, and error prone (Torri, A. F., Kunkel, T. A., and Englund, P. T. (1994) J. Biol. Chem. 269, 8165-8171), In allof these characteristics, the enzyme resembles DNA polymerase beta, anuclear enzyme thought to be involved in DNA repair, We have now cloned and sequenced the gene for this enzyme. The mitochondrial polymerase has significant homology, about 33% identity at the amino acid level, with human DNA polymerase beta. However, sequence analysis of the clone revealed the presence of a cleaved N-terminal presequence, presumably a mitochondrial import signal, which resembles presequences on other C. fasciculata mitochondrial proteins, The polymerase's function may be to repair the many gaps in newly replicated kinetoplast (mitochondrial) DNA minicircles in this parasite, This enzyme is the first example of a mitochondrial DNA polymerase beta.

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Documento generato il 03/12/20 alle ore 21:35:58