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Titolo:
DEVELOPMENT OF VENTRAL COCHLEAR NUCLEUS PROJECTIONS TO THE SUPERIOR OLIVARY COMPLEX IN GERBIL
Autore:
KIL J; KAGEYAMA GH; SEMPLE MN; KITZES LM;
Indirizzi:
UNIV CALIF IRVINE,DEPT ANAT & NEUROBIOL IRVINE CA 92717 UNIV CALIF IRVINE,DEPT ANAT & NEUROBIOL IRVINE CA 92717
Titolo Testata:
Journal of comparative neurology
fascicolo: 3, volume: 353, anno: 1995,
pagine: 317 - 340
SICI:
0021-9967(1995)353:3<317:DOVCNP>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
AUDITORY BRAIN-STEM; TMB REACTION-PRODUCT; D-ASPARTIC ACID; HORSERADISH-PEROXIDASE; INFERIOR COLLICULUS; MONGOLIAN GERBIL; DENDRITIC MORPHOLOGY; MERIONES-UNGUICULATUS; TRAPEZOID BODY; GUINEA-PIG;
Keywords:
AUDITORY BRAIN-STEM; LATERAL SUPERIOR OLIVE; CALYXES OF HELD; DII; PHOTOOXIDATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
79
Recensione:
Indirizzi per estratti:
Citazione:
J. Kil et al., "DEVELOPMENT OF VENTRAL COCHLEAR NUCLEUS PROJECTIONS TO THE SUPERIOR OLIVARY COMPLEX IN GERBIL", Journal of comparative neurology, 353(3), 1995, pp. 317-340

Abstract

The postnatal development of the projection from the ventral cochlearnucleus to the principal nuclei in the superior olivary complex in gerbil (Meriones unguiculatus) was studied in an age-graded series of pups ranging from 0 to 18 days old. Small crystals of -dioctadecyl-3,3,3';3'-tetramethylindocarbocyanine perchlorate (DiI) were inserted into the ventral cochlear nucleus of aldehyde-fixed brains, and the labeledprojections were examined with epifluorescence microscopy, Selected sections were photooxidized in a solution of diaminobenzidine and subsequently processed for electron microscopy to examine the development of labeled synapses in the target nuclei. Horseradish peroxidase was injected into the ventral cochlear nucleus of adult gerbils to assess the form and persistence of projections observed in the neonatal animals. In addition, electrophysiological responses to acoustic stimuli of single units in the adult auditory brainstem were analyzed to confirm the functionality of the novel projection from the ventral cochlear nucleus to the contralateral lateral superior olive. By the day of birth (PO), developing axons from the ventral cochlear nucleus have already established highly ordered pathways to the three primary nuclei of thesuperior olivary complex: the ipsilateral lateral superior olive, thecontralateral medial nucleus of the trapezoid body, and at the lateral and medial dendrites of the ipsilateral and contralateral medial superior olive, respectively. Developing axons from the ventral cochlear nucleus that innervated the contralateral medial nucleus of the trapezoid body lacked the terminal morphology characteristic of the calyx ofHeld, but began to adopt a more characteristic form on P5. The maturecalyx appeared around P14-16, Exuberant developmental projections to topographically inappropriate areas of the superior olivary complex were not observed at the postnatal ages studied. In addition to the projections of the ventral cochlear nucleus to the superior olivary complex described in other species, we observed the development and maintenance of a major direct projection from the ventral cochlear nucleus to the contralateral lateral superior olive. On PO, ventral cochlear nucleus axons decussate;in the dorsal trapezoid body, form a plexus at thedorsal edge of the contralateral medial superior olive, and enter theventrolateral limb of the contralateral lateral superior olive. Over the next 2 weeks, fascicles of fibers form on the hilar and ventral aspects of the ventrolateral limb. Fibers arising from these fascicles form converging, but nonoverlapping, arborizations within the ventrolateral limb at right angles-to the curvature of the nucleus. The medial region was devoid of labeled axons. The direct innervation of the contralateral lateral superior olive was confirmed in the adult gerbil with anterograde horseradish peroxidase histochemistry and by the recording of excitatory responses in the innervated region to acoustic stimulation of the contralateral ear. (C) 1995 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:41:23