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Titolo:
GENE-TARGETED INHIBITION OF TRANSACTIVATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1)-LTR BY ANTISENSE OLIGONUCLEOTIDES
Autore:
DEMIRHAN I; HASSELMAYER O; HOFMANN D; CHANDRA A; SVINARCHUK FP; VLASSOV VV; ENGELS J; CHANDRA P;
Indirizzi:
UNIV FRANKFURT,SCH MED,MOLEC BIOL LAB,THEODOR STERN KAI 7 D-60590 FRANKFURT GERMANY UNIV FRANKFURT,SCH MED,MOLEC BIOL LAB D-60590 FRANKFURT GERMANY RUSSIAN ACAD SCI,INST BIOORGAN CHEM NOVOSIBIRSK 630090 RUSSIA UNIV FRANKFURT,INST ORGAN CHEM W-6000 FRANKFURT GERMANY
Titolo Testata:
Virus genes
fascicolo: 2, volume: 9, anno: 1995,
pagine: 113 - 119
SICI:
0920-8569(1995)9:2<113:GIOTOH>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONICALLY INFECTED-CELLS; TAT PROTEIN; PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDE; REVERSE TRANSCRIPTION; SELECTIVE-INHIBITION; INTERCALATING AGENT; KAPOSIS-SARCOMA; MESSENGER-RNA; REPLICATION; PROLIFERATION;
Keywords:
TRANSACTIVATION; TAT EXPRESSION; ANTISENSE OLIGONUCLEOTIDES; ANTI-TAT-IGG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
I. Demirhan et al., "GENE-TARGETED INHIBITION OF TRANSACTIVATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1)-LTR BY ANTISENSE OLIGONUCLEOTIDES", Virus genes, 9(2), 1995, pp. 113-119

Abstract

We have used an in vitro approach to study the efficiency of antisense oligonucleotides in inhibiting LTR-(HIV-1)-directed CAT expression catalyzed by tat protein, the functional protein of the transactivator gene. We selected the target sequence localized near the 5' end of thetat mRNA. The following conclusions can be drawn from the data presented here: a) Antisense oligonucleotides modified by conjugation of cholesterol at the 3' end have a severalfold higher inhibitory response, b) inhibitory response is dependent on the mode of introducing oligonucleotides, and c) the inhibition by antisense oligonucleotides is sequence specific and directed towards the targeted region. This approach could be useful for targeting functional regions of regulatory gene products and designing gene-targeted inhibitors of virus replication.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 06:37:54