Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MEASUREMENT OF CLOZAPINE AND NORCLOZAPINE IN PLASMA SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION
Autore:
MCCARTHY PT; HUGHES S; PATON C;
Indirizzi:
ST THOMAS HOSP,CTR HAEMOPHILIA,1ST FLOOR N WING LONDON SE1 7EH ENGLAND GUYS & ST THOMAS NHS TRUST,NATL POISONS UNIT LONDON SE14 5ER ENGLAND BEXLEY HOSP,DEPT PHARM BEXLEY DA5 2BW KENT ENGLAND
Titolo Testata:
BMC. Biomedical chromatography
fascicolo: 1, volume: 9, anno: 1995,
pagine: 36 - 41
SICI:
0269-3879(1995)9:1<36:MOCANI>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
PH BUFFER ELUENT; BASIC DRUGS; RETENTION REPRODUCIBILITY; SILICA COLUMN; HUMAN-SERUM; PHASE; DESMETHYLCLOZAPINE; METABOLITES; SEPARATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
P.T. Mccarthy et al., "MEASUREMENT OF CLOZAPINE AND NORCLOZAPINE IN PLASMA SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION", BMC. Biomedical chromatography, 9(1), 1995, pp. 36-41

Abstract

A simple method for the measurement of clozapine and its N-desmethyl metabolite in human plasma or serum by high performance liquid chromatography is described. An internal standard (aqueous nortriptyline, 4 mg/L) (50 mu L) and Tris buffer (2 mol/L, pH 10.6) (100 mu L) are addedto plasma/serum (200 pi) and the analytes and internal standard extracted into methyl tert-butyl ether (200 mu L). The extracts are analysed on a 150 mm column containing Spherisorb S5SCX using methanol containing ammonium perchlorate (35 mmol/L, pH 6.7) as eluent at a flow rateof 1.5 mL/min. Detection is by ultraviolet absorption (215 nm). The limit of detection is better than 0.05 mg/L for both analytes and the intra-assay precision (CV) for clozapine and norclozapine was 5.3 and 7.3% at 0.5 mg/L and 2.6 and 2.8% at 1.5 mg/L, respectively. The methodcan be applied to the measurement of these compounds in plasma after acute overdosage, for the assessment of compliance in patients apparently refractory to therapy and to identify interactions between clozapine and other neuroleptic and antidepressant drugs which may effect toxicity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 01:56:59