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Titolo:
REGULATION OF THE ACETYLCHOLINE-RECEPTOR EPSILON-SUBUNIT GENE BY RECOMBINANT ARIA - AN IN-VITRO MODEL FOR TRANSYNAPTIC GENE-REGULATION
Autore:
CHU GC; MOSCOSO LM; SLIWKOWSKI MX; MERLIE JP;
Indirizzi:
WASHINGTON UNIV,SCH MED,DEPT MOLEC BIOL & PHARMACOL ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT ANAT & NEUROBIOL ST LOUIS MO 63110 GENENTECH INC S SAN FRANCISCO CA 94080
Titolo Testata:
Neuron
fascicolo: 2, volume: 14, anno: 1995,
pagine: 329 - 339
SICI:
0896-6273(1995)14:2<329:ROTAEG>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
VERTEBRATE NEUROMUSCULAR-JUNCTION; PREFERENTIAL SYNAPTIC EXPRESSION; CULTURED MUSCLE-CELLS; RAT SOLEUS MUSCLE; MESSENGER-RNA; ALPHA-SUBUNIT; MOUSE MUSCLE; INSITU HYBRIDIZATION; TRANSGENIC MICE; BETA-SUBUNIT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
68
Recensione:
Indirizzi per estratti:
Citazione:
G.C. Chu et al., "REGULATION OF THE ACETYLCHOLINE-RECEPTOR EPSILON-SUBUNIT GENE BY RECOMBINANT ARIA - AN IN-VITRO MODEL FOR TRANSYNAPTIC GENE-REGULATION", Neuron, 14(2), 1995, pp. 329-339

Abstract

Structural specialization of the postsynaptic skeletal muscle membrane is in part mediated by the motor neuron-induced transcriptional regulation of synaptic muscle nuclei. ARIA, a factor that stimulates production of acetylcholine receptors (AChRs), is a candidate signaling molecule for such regulation. Here we examine the transynaptic inducing potential of this polypeptide factor. ARIA immunoreactivity is detectable at synaptic sites in vivo. In vitro, recombinant heregulin beta 1 (rHRG beta 1), the human homolog of ARIA, induces expression of the AChR epsilon gene, the subunit most sensitive to synaptic input. The inducing property of rHRG beta 1 is demonstrated most dramatically in primary muscle cultures from transgenic mice bearing an epsilon promoter-nuclear lacZ reporter transgene. transient transfection experiment; using the Sol 8 muscle cell line indicate that sequences that confer responsiveness to ARIA are located within a 150 bp epsilon subunit promoter region and are, E box-independent. These results suggest that ARIA performs a vital role by directing spatially restricted gene expressionat the neuromuscular junction.

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Documento generato il 24/11/20 alle ore 12:42:11