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Titolo:
THE ELECTROPHORETIC POLYMORPHISM OF BACTERIAL ESTERASES
Autore:
GOULLET P; PICARD B;
Indirizzi:
HOP BEAUJON,MICROBIOL LAB,100 BD GEN LECLERC F-92110 CLICHY FRANCE UNIV PARIS 07,FAC MED XAVIER BICHAT,MICROBIOL LAB F-75018 PARIS FRANCE
Titolo Testata:
FEMS microbiology reviews
fascicolo: 1, volume: 16, anno: 1995,
pagine: 7 - 31
SICI:
0168-6445(1995)16:1<7:TEPOBE>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
FRAGMENT-LENGTH-POLYMORPHISM; ISOELECTRIC FOCUSING-ELECTROPHORESIS; POLYNUCLEOTIDE SEQUENCE RELATEDNESS; POLYACRYLAMIDE-GEL ELECTROPHORESIS; DEOXYRIBONUCLEIC-ACID RELATEDNESS; METHICILLIN-RESISTANT STRAINS; RIBOSOMAL DNA POLYMORPHISM; PROTEIN TITRATION CURVES; ESCHERICHIA-COLI STRAINS; CARBOXYLESTERASE-B;
Keywords:
ESTERASES; ELECTROPHORETIC POLYMORPHISM; SYSTEMATICS; EPIDEMIOLOGY; POPULATION GENETICS; PHYLOGENY;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
194
Recensione:
Indirizzi per estratti:
Citazione:
P. Goullet e B. Picard, "THE ELECTROPHORETIC POLYMORPHISM OF BACTERIAL ESTERASES", FEMS microbiology reviews, 16(1), 1995, pp. 7-31

Abstract

The specific activities of esterases and certain other molecular properties including immunospecificity indicate that the electrophoretic variations of these enzymes in bacterial populations are the result of allelic variations at specific gene loci. The esterase polymorphism ofEnterobacteriaceae and some other species isolated from man or animals demonstrates that esterases can distinguish between bacteria at the species or subspecies level, both by their biochemical properties and by their electrophoretic differences. The esterase data complement DNAhybridization studies and agree with ribosomal DNA polymorphism, especially for delineating a phylogenetically distinct group of highly pathogenic strains in Escherichia coli. A two-dimensional electrophoreticprofile obtained by establishing a direct correspondence between homologous esterase bands resolved by independent runs of isoelectric focusing and standard electrophoresis considerably improves the detection of allelic variations, whereas protein titration curves (electrophoresis in pH gradient) can be used to demonstrate the real electrophoretichomogeneity of allozymes or evalue their molecular relationship in terms of apparent amino acid substitutions. This overview establishes that esterases, by their significant electrophoretic polymorphism, are reliable molecular markers for systematics and epidemiology, and are suitable enzyme systems for studying population genetics and phylogeny.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 07:47:55