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Titolo:
GENETIC-CHARACTERIZATION OF THE HUMAN PAPILLOMAVIRUS (HPV) 18-E2 GENEIN CLINICAL SPECIMENS SUGGESTS THE PRESENCE OF A SUBTYPE WITH DECREASED ONCOGENIC POTENTIAL
Autore:
HECHT JL; KADISH AS; JIANG G; BURK RD;
Indirizzi:
ALBERT EINSTEIN COLL MED,DEPT MICROBIOL & IMMUNOL,ULLMANN BLDG,RM 625,1300 MORRIS PK AVE BRONX NY 10461 ALBERT EINSTEIN COLL MED,DEPT MICROBIOL & IMMUNOL BRONX NY 10461 ALBERT EINSTEIN COLL MED,DEPT PATHOL BRONX NY 10461 ALBERT EINSTEIN COLL MED,DEPT PEDIAT BRONX NY 10461
Titolo Testata:
International journal of cancer
fascicolo: 3, volume: 60, anno: 1995,
pagine: 369 - 376
SICI:
0020-7136(1995)60:3<369:GOTHP(>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; CERVICAL NEOPLASIA; PHYSICAL STATE; TYPE-16; CANCER; DNA; CARCINOMA; PROTEINS; LESIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
J.L. Hecht et al., "GENETIC-CHARACTERIZATION OF THE HUMAN PAPILLOMAVIRUS (HPV) 18-E2 GENEIN CLINICAL SPECIMENS SUGGESTS THE PRESENCE OF A SUBTYPE WITH DECREASED ONCOGENIC POTENTIAL", International journal of cancer, 60(3), 1995, pp. 369-376

Abstract

HPV 18 is associated with 2 divergent phenotypes: (ii aggressive cervical cancer and a preponderance of cancer relative to cervical intra-epithelial neoplasia (GIN) and (ii) benign warty lesions of the cervix. The E2 gene of HPV 18 encodes a regulatory protein that represses viral oncogene transcription and is involved in viral replication. Variation within the E2 gene of HPV 18 and its correlation with the morphologic grade of associated lesions were analyzed in a sample of 20 HPV 18-positive cervical specimens representing a spectrum of pathology fromlow-grade CIN to cervical cancer. An amplifiable HPV 18 E2 gene was present in 3 of 5 cancers, indicating that E2 disruption was not required far cancer development. Single-strand conformation polymorphism andPCR analyses revealed a high degree of polymorphism throughout the E2gene. Direct DNA sequencing of both strands of a 154-bp fragment revealed a variability of 5.8%. Six intra-epithelial lesions contained alterations in common that account for 3.9% of the variation and appear to constitute a subtype. Within the 154-bp region, 2 of 3 cervical cancers and 0 of 12 intra-epithelial lesions were identical to the published HPV 18 sequence. DNA sequence analysis of a region extending into the E5 open reading frame revealed deletions in the E2/E5 intragenic region that were present in 50% of the members of the subtype. Our data demonstrate significant sequence variation within the E2 gene and suggest the presence of an HPV 18 subtype with decreased oncogenic potential. (C) 1995 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 06:39:51