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Titolo:
REGULATION OF MONOCYTE CHEMOTACTIC PROTEIN-1 GENE-EXPRESSION IN HUMANENDOMETRIAL CELLS IN CULTURES
Autore:
ARICI A; MACDONALD PC; CASEY ML;
Indirizzi:
UNIV TEXAS,SW MED CTR,CECIL H & IDA GREEN CTR REPROD BIOL SCI,5323 HARRY HINES BLVD DALLAS TX 75235 UNIV TEXAS,SW MED CTR,CECIL H & IDA GREEN CTR REPROD BIOL SCI DALLAS TX 75235 UNIV TEXAS,SW MED CTR,DEPT OBSTET GYNECOL DALLAS TX 75235 UNIV TEXAS,SW MED CTR,DEPT BIOCHEM DALLAS TX 75235
Titolo Testata:
Molecular and cellular endocrinology
fascicolo: 2, volume: 107, anno: 1995,
pagine: 189 - 197
SICI:
0303-7207(1995)107:2<189:ROMCPG>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; BLOOD MONONUCLEAR LEUKOCYTES; CYTOKINE-INDUCED PRODUCTION; AMINO-ACID ANALYSIS; CHEMOATTRACTANT PROTEIN-1; ENDOTHELIAL-CELLS; ACTIVATING FACTOR; GROWTH-FACTOR; HUMAN-FIBROBLASTS; EPITHELIAL-CELLS;
Keywords:
ENDOMETRIAL CELLS; MONOCYTE CHEMOTACTIC PROTEIN-1; REGULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
A. Arici et al., "REGULATION OF MONOCYTE CHEMOTACTIC PROTEIN-1 GENE-EXPRESSION IN HUMANENDOMETRIAL CELLS IN CULTURES", Molecular and cellular endocrinology, 107(2), 1995, pp. 189-197

Abstract

Bone marrow-derived leukocytes are present in human endometrium/decidua and are believed to serve a variety of functions in this tissue. The number and type of leukocytes in endometrium/decidua vary with the hormonal milieu of the ovarian cycle, with blastocyst implantation, andduring pregnancy. The factors that regulate the recruitment of specific leukocytes to the endometrium and those that modulate the function or replication of leukocytes in this tissue are not well defined. In this study, we evaluated the potential for synthesis of monocyte chemotactic protein-1 (MCP-1), a polypeptide with monocyte/macrophage chemotactic and activating properties, in human endometrium and in separatedendometrial stromal and epithelial cells. MCP-1 mRNA was readily detected by northern analysis of total RNA isolated from human endometrialtissue (n= 39 tissues from ovulatory women; n = 3 atrophic endometriafrom anovulatory women; n = 6 from women ingesting oral contraceptives or medroxyprogesterone acetate) and decidua parietalis at midtrimester (n = 6 pregnancies) and at term (n = 6 pregnancies). The levels of MCP-1 mRNA varied considerably among tissues; but in this relatively small number of samples, there was no apparent relationship between dayof cycle, endocrine status, or duration of pregnancy and the level ofMCP-1 mRNA. MCP-1 mRNA was detected in separated endometrial stromal cells and epithelial cells in culture. In confluent human endometrial stromal cells in the absence or presence of fetal bovine serum (10%, v/v), MCP-1 mRNA was detected by northern analysis of total RNA. Fetal bovine serum effected a concentration-dependent decrease in the levelsof MCP-1 mRNA. The level of MCP-1 mRNA in endometrial stromal cells maintained in serum-free medium increased in a time- and concentration-dependent manner in response to treatment with interleukin-1 alpha or tumor necrosis factor-alpha. Platelet-derived growth factor and interferon-alpha also acted, in a concentration-dependent manner, to increase the level of MCP-1 mRNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 20:42:14