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Titolo:
FUNCTIONALLY DISTINCT HUMAN MARROW STROMAL CELL-LINES IMMORTALIZED BYTRANSDUCTION WITH THE HUMAN PAPILLOMA-VIRUS E6 E7 GENES/
Autore:
ROECKLEIN BA; TOROKSTORB B;
Indirizzi:
FRED HUTCHINSON CANC RES CTR,DIV CLIN RES,TRANSPLANTAT BIOL PROGRAM,1124 COLUMBIA ST SEATTLE WA 98104
Titolo Testata:
Blood
fascicolo: 4, volume: 85, anno: 1995,
pagine: 997 - 1005
SICI:
0006-4971(1995)85:4<997:FDHMSC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-BONE-MARROW; HEMATOPOIETIC STEM-CELLS; PROGENITOR CELLS; ADHERENT CELLS; CULTURES; PROLIFERATION; DIFFERENTIATION; PURIFICATION; EXPRESSION; INVITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
B.A. Roecklein e B. Torokstorb, "FUNCTIONALLY DISTINCT HUMAN MARROW STROMAL CELL-LINES IMMORTALIZED BYTRANSDUCTION WITH THE HUMAN PAPILLOMA-VIRUS E6 E7 GENES/", Blood, 85(4), 1995, pp. 997-1005

Abstract

A replication-defective recombinant retrovirus containing the human papilloma virus E6/E7 genes (LXSN-16 E6E7) was used to immortalize stromal cells from human marrow. The E6/E7 gene products interfere with the function of tumor-suppressor proteins p53 and Rb, respectively, thereby preventing cell cycle arrest without causing significant transformation. Twenty-seven immortalized clones designated HS-1 to HS-27 were isolated, four of which are characterized in this report. Two cell lines, HS-5 and HS-21, appear to be fibroblastoid and secrete significantlevels of granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage-CSF (GM-CSF), macrophage-CSF (M-CSF), Kit ligand (KL), macrophage-inhibitory protein-1 alpha, interleukin-6 (IL-6), IL-8, and IL-11. However, only HS-5 supports proliferation of hematopoietic progenitor cells when cocultured in serum-deprived media with no exogenous factors. Conditioned media (CM) from HS-5 promotes growth of myeloid colonies to significantly greater extent than a cocktail of recombinant factors containing 10 ng/mL of IL-1, IL-3, IL-6, G-CSF, GM-CSF, and KL and 3 U of erythropoietin (Epo). Two additional clones, HS-23 and HS-27, resemble ''blanket'' cells, with an epithelioid morphology, and aremuch larger, broader, and flatter when compared with HS-5 and HS-21. These lines secrete low levels of growth factors and do not support proliferation of isolated progenitor cells in cocultures. CM from HS-23 and HS-27 also fail to support growth of myeloid colonies. Both HS-23 and HS-27 express relatively high levels of VCAM-1, yet HS-27 is the only line that supports the formation of ''cobblestone'' areas by isolated CD34(+)38(lo) cells. We hypothesize that HS-5, HS-21, HS-23, and HS-27 represent functionally distinct components of the marrow microenvironment. (C) 1995 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:19:33